甘蔗乙烯受体Sc-ERS启动子的克隆与序列分析  

Cloning and sequence analysis of promoter in sugarcane ethylene receptor gene(Sc-ERS)

作　　者：黄静丽[1] 牛俊奇[1] 朱惠[1] 杨丽涛[1] 李杨瑞[1,2] 王爱勤[1,2] 

机构地区：[1]广西大学农学院,亚热带农业生物资源保护与利用国家重点实验室,南宁530005 [2]中国农业科学院甘蔗研究中心/广西农业科学院甘蔗研究所,农业部广西甘蔗生物技术与遗传改良重点实验室,广西甘蔗遗传改良重点实验室,南宁530007

出　　处：《南方农业学报》2013年第5期722-729,共8页Journal of Southern Agriculture

基　　金：国家“863”计划项目(2013AA102604);国际科技合作项目(2009DFA30820,2013DFA31600);广西自然科学基金项目(2011GXNSFA018069,2011GXNSFF018002);广西自治区主席科技资金项目(11166-02)

摘　　要：【目的】克隆甘蔗乙烯受体Sc-ERS基因启动子序列,分析Sc-ERS基因启动子序列的作用元件。【方法】采用基于热不对称交错式PCR原理的染色体步移技术,从甘蔗ROC22基因组DNA中克隆Sc-ERS基因启动子序列,利用启动子预测软件PlantCARE和PLACE在线工具对Sc-ERS启动子序列的作用元件进行预测。【结果】克隆获得Sc-ERS启动子序列1410bp,该序列与玉米ERS25、ERS14核苷酸同源性分别为82%和80%。PlantCARE在线分析结果表明,该序列具有启动子基本作用元件TATA-BOX和CAAT-BOX,还含有参与光响应元件、干旱诱导MYB结合位点、茉莉酸甲酯响应元件、水杨酸响应元件、胚乳表达顺式调控元件、热胁迫响应元件等。【结论】从甘蔗基因组DNA中克隆获得乙烯受体Sc-ERS基因上游1410bp的启动子序列,该序列含有多个特异性调控元件,推测Sc-ERS基因的表达可能受生理周期、激素、干旱、光照等因素调控。[Objective]The present study was conducted to clone the promoter sequence of sugarcane ethylene receptor （Sc-ERS） and analyze its acting elements. [Method]Sc-ERS promoter sequence was cloned from the genomic DNA of sug- arcane cuhivar ROC22 with chromosome walking technology based on thermal asymmetric interlaced PCR principle, and the acting elements of Sc-ERS were analyzed with PlantCARE and PLACE online tools. [Result]The gene promoter sequence of Sc-ERS was cloned, and it was 1410 bp in size sharing 82% and 80% homology with the sequences of ERS25 and ERS14 of corn, respectively. PlantCARE online analysis results showed that the gene promoter sequence of Sc-ERS had promoter acting elements TATA-BOX and CAAT-BOX, light-responsive elements, drought-induced MYB binding sites, methyl jasmonate- responsive element, salicylic acid response element, endosperm expression cis-regulatory elements, heat stress responsive el- ements and so on. [Conclusion]A 1410 bp promoter sequence of Sc-ERS was cloned from sugarcane genomic DNA, which con- tained several specific acting elements, indicating that its expression could be regulated by circadian rhythm, hormone, drought, light and other factors.

关 键 词：甘蔗 乙烯受体基因 启动子序列 克隆 

分 类 号：S566.1[农业科学—作物学]