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机构地区:[1]南宁学院,南宁530200 [2]广西大学动物科学技术学院,南宁530005
出 处:《南方农业学报》2013年第5期850-853,共4页Journal of Southern Agriculture
基 金:国家科技支撑计划项目(2012BAD25B04);国家自然科学基金项目(31260640);广西高等学校科研项目(201204LX670)
摘 要:【目的】测定红罗非鱼(Oreochromis sp.)稚鱼、雌雄亲鱼的肌肉脂肪酸组成,为红罗非鱼的生物学研究及科学养殖提供基础资料。【方法】采用常规的化学分析方法及气相色谱技术检测红罗非鱼稚鱼与雌雄亲鱼肌肉脂肪酸的组成及含量变化。【结果】从红罗非鱼稚鱼肌肉中检测出10种脂肪酸,雌雄亲鱼肌肉中检测出16种脂肪酸,以C16:0、C16:1、C18:0、C18:1n-9、C18:2n-6的含量较高,占总脂肪酸含量的84%以上。红罗非鱼肌肉脂肪酸含量因生长期和性别不同而异,C20:5n-3(EPA)和C22:6n-3(DHA)在稚鱼肌肉中未能检出,雌亲鱼肌肉中含有少量(0.47%h和0.65%),雄亲鱼肌肉含量丰富(1.39%和2.33%);红罗非鱼稚鱼和雄性亲鱼肌肉中,脂肪酸含量次序为:饱和脂肪酸含量(ΣSFA)>单不饱和脂肪酸含量(ΣMUFA)>多不饱和脂肪酸含量(ΣPUFA),雌性亲鱼肌肉中:ΣMUFA>ΣSFA>ΣPU-FA。【结论】红罗非鱼稚鱼阶段主要依靠ΣSFA提供能量以满足其生长所需,雌雄亲鱼则依靠ΣMUFA和ΣPUFA来提高其繁殖性能。[ Objective ] Fatty acid compositions in muscle of fry, male and female parent fish (Oreochromis sp. ) were determined to provide references for biological research and scientific breeding of Oreochromis sp.. [Method]The fatty acid composition and content in muscle of fry, female and male parent fish were analyzed using conventional chemical method and GC technology. [Result]The results showed that there were ten kinds of fatty acid in muscle of fry, and sixteen kinds of fatty acid in muscle of parent fish. C16:0,C16:1 ,C18:0,C18:1n-9 and C18:2n-6 (ARA) took up more than 84% of total fatty acid. Fatty acid content in muscle of red tilapia was affected by different growth stages and genders. C20:5n-3 (EPA) and C22:6n-3 (DHA) were not found in muscle of fry fish, but little in female parent fish (0.47% and 0.65%), and much in male parent fish (1.39% and 2.33%). Fatty acid content showed the following descending sequence of ESFA〉 EMUFA〉EPUFA in muscle of fry red tilapia and male parent fish, and EMUFA〉~SFA〉EPUFA in muscle of red tilapia fe- male parent fish. [ Conclusion ]Fry red tilapia mainly lives on ESFA, while male and female parent fish need EMUFA and EPUFA to improve reproductive performance.
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