酶促合成氨基酸神经肽的前体二肽  

Synthesis of A Precursor Dipeptide of Neural Tetrapeptide by Enzyme

作　　者：李世军[1] 李彦文[1] 王树民 杨梅[1] 李力群[1] 温钢[1] 展瑞岩[1] 

机构地区：[1]吉林化工学院环境与生物工程学院,吉林吉林132022 [2]苏州金盟生物技术有限公司,太仓江苏215421

出　　处：《吉林化工学院学报》2013年第5期29-32,共4页Journal of Jilin Institute of Chemical Technology

摘　　要：本研究在非水介质中酶法合成了氨基酸神经四肽Phe-Arg-Trp-Gly(FRWG)的前体二肽BOC-Phe-Arg-OMe,胰凝乳蛋白酶(ChyT)作为催化剂在水-有机溶剂体系中催化合成目标二肽.通过考查有机溶剂种类、水含量、温度、pH等对产物二肽BOC-Phe-Arg-OMe的影响,优化了合成反应的条件.并确定了最佳反应条件:使用胰凝乳蛋白酶作为催化剂,在pH为7.5,温度40℃,二甲基甲酰胺(DMF)/Tris-HCl缓冲液(98.5 1.5,V/V),反应8 h,二肽产量达到61.25%.The protease-catalyzed synthesis of a precursor dipeptide of neural tetrapeptide （ Phe-Arg-Trp-Gly）, BOC-Phe-Arg-OMe in non-aqueous media was studied. An chymotrypsin was used to catalyze the synthesis of the target dipeptide in water-organic cosolvents systems. The conditions of the synthesis reaction were optimized by examining the effects of several factors including organic solvents, water content, temperature, and pH in DMF/Tris-HCl buffer system （98.5 ： 1.5 ,V/V） ,8h with the dipeptide yield of 61.25%.

关 键 词：肽合成 蛋白酶 催化 有机溶剂 

分 类 号：Q559.3[生物学—生物化学]