戊地昔布联用吡柔比星对人肺癌A549细胞凋亡的影响  

Effect of Combination Valdecoxib and Pirarubicin on Promoting Apoptosis in Human Lung Cancer Cell

作　　者：徐清华[1] 侯晓敏[2] 王慧慈[3] 于永燕 李军霞[3] 

机构地区：[1]廊坊市人民医院药剂科,河北廊坊065000 [2]河北医科大学研究生学院,石家庄050000 [3]河北医科大学药理教研室,石家庄050000 [4]石家庄市河东社区卫生服务中心,石家庄050000

出　　处：《中国药学杂志》2013年第12期972-975,共4页Chinese Pharmaceutical Journal

基　　金：河北省科技攻关资助项目(07276403D);河北省卫生厅医学重点课题(2008067)

摘　　要：目的观察戊地昔布与吡柔比星联合应用对人肺癌A549细胞的抑制效应、细胞周期和凋亡的影响。方法 用四甲基偶氮唑蓝法检测戊地昔布与吡柔比星对人肺癌A549细胞的抑制率,并用中效原理判断二者之间相互作用;用流式细胞术检测肿瘤细胞周期分布和细胞凋亡率;用蛋白质印迹法检测Bcl-2、Bax和caspase-3等凋亡蛋白表达。结果 戊地昔布与吡柔比星联合用药对人肺癌A549细胞的抑制率比二者单独用药时明显增加,合用指数值小于1,细胞凋亡率也明显提高。联合用药后,Bcl-2表达进一步减少、Bax和caspase-3表达进一步增多。结论戊地昔布和吡柔比星联合用药后可通过增加细胞凋亡率而明显增强对A549细胞生长的抑制作用。OBJECTIVE To study the effect of valdecoxib and pirarubicina combination on cell cycle and apoptosis of human lung cancer A549 cell line in vitro. METHODS MTF assay was used to analyze the effect of valdecoxib and pirarubicina on the growth of human lung cancer A549 cell line. The median-effect principle was applied to determine the combination effect of valdecoxib and pirarubicina. Flow cytometry （FCM） was used to observe the cell cycle and apoptosis. The expressions of Bcl-2, Bax, and Caspase-3 were detected by western blotting. RESULTS The inhibition ratio of A549 cell in valdecoxib and pirarubicina combination group was increased compared with their respective application, and CI 〈 1. In valdecoxib and pirarubicina combination group, the apoptosis rate and the expression of Bax and Caspase-3 was increased, and the expression of Bcl-2 was decresed. CONCLUSION Valdecoxib and pirarubicina combination has synergistic effect,which is partly due to the increase of the apoptosis rate.

关 键 词：戊地昔布 人肺癌A549 凋亡 吡柔比星 

分 类 号：R965[医药卫生—药理学]