高效毛细管电泳法同时测定牛樟芝中5种核苷类成分的含量  

Simultaneous Determination of Five Nucleosides in Antrodia camphorata by HPCE

作　　者：张奉苏[1] 陈菲[1] 傅兴圣[1] 刘训红[1] 杨念云[1] 蔡宝昌[1] 夏敏媛[1] 

机构地区：[1]南京中医药大学江苏省中药炮制重点实验室,南京210046

出　　处：《中国药学杂志》2013年第12期1018-1021,共4页Chinese Pharmaceutical Journal

基　　金：江苏省中药炮制重点实验室开放式课题(ZYPZ007);江苏省高校优秀科技创新团队"中药资源化学研究"(2011);江苏高校优势学科建设工程资助项目(ysxk-2010)

摘　　要：目的建立高效毛细管电泳法(HPCE)同时测定不同批次牛樟芝中腺嘌呤、腺苷、尿苷、鸟苷和肌苷等5种核苷类成分含量的方法。方法 采用未涂渍标准熔融石英毛细管(75μm×64.5 cm,有效长度56 cm)为分离通道,60 mmol.L-1硼砂(pH 9.3)为运行缓冲液,分离电压为22 kV,检测波长为260 nm,毛细管温度为28℃,压力进样为50 mbar×6 s。结果 5种核苷类成分的响应峰面积与其相应浓度的线性关系良好(r>0.999 5);加样回收率为98.83%～101.08%。实验表明,不同批次牛樟芝菌粉中5种核苷类成分的含量有所差异。结论该方法准确、可靠,重复性较好,可用于牛樟芝菌粉内在质量的评价和控制。OBJECTIVE To establish a high performance capillary electrophoresis （HPCE） method for simultaneous determination of five nueleosides and nueleobases, including adenine, adenosine, uridine, guanosine and inosine in antrodia camphorate of different batches. METHODS Based on the mode of HPCE, uncoated fused silica capillary （ 75 μm × 64. 5 cm , 56 cm of effective length） was used with separation voltage of 22 kV. 60 mmol . L^-1 sodium borate was selected for the running buffer solution （ pH 9. 3）. The deteetion wavelength was set at 260 nm. The sample was injected at 50 mbar × 6 s and column temperature was maintained at 28 ℃. RESULTS The calibration curves of the five nueleosides showed good linearity （r 〉 0. 999 5）. The average recoveries of the method were between 98. 83% - 101.08%. The contents of the five nucleosides in Antrodia camphorate samples of different batches were different. CONCLUSION The established method is reliable, accurate and can be used for the quality control of Antrodia camphorata.

关 键 词：高效毛细管电泳 牛樟芝 核苷 碱基 同时测定 

分 类 号：R917[医药卫生—药物分析学]