参麦注射液对环孢霉素A肾毒性的拮抗作用及机制探讨  

Antagonistic effect and its mechanism of Shenmai injection on nephrotoxicity induced by cyclosporin A

作　　者：黄珀[1] 张春梅[1] 陈蓉[1] 万英[1] 张春燕[1] 邓勇[2] 

机构地区：[1]泸州医学院病理生理学教研室,四川泸州646000 [2]泸州医学院附属医院泌尿外科

出　　处：《现代预防医学》2013年第12期2337-2338,2346,共3页Modern Preventive Medicine

摘　　要：目的探讨参麦注射液预防环孢霉素A中毒性肾损伤的机制。方法实验大鼠分对照组、环孢霉素A中毒组及参麦注射液处理组,分别给予橄榄油灌胃,环孢霉素A灌胃,环孢霉素A灌胃同时参麦注射液腹腔注射,连续4周后,取肾组织作病理观察,并测肾组织超氧化物歧化酶和丙二醛含量,取血测血肌酐、尿素氮和血流变学指标。结果与对照组相比,环孢霉素A组大鼠,肾脏功能明显受损,血肌酐、尿素氮明显增高;血液呈高黏滞状态,全血黏度和红细胞压积显著降低,血浆黏度,红细胞聚集指数,纤维蛋白原显著增高;肾组织氧化性损伤加重,超氧化物歧化酶含量减低而氧化产物丙二醛MDA含量增高;与环孢霉素A中毒组相比,参麦注射液组大鼠肾脏功能受损明显减轻,血肌酐、尿素氮降低,脂质过氧化损伤减轻,血流变各项指标均有明显改善。结论参麦注射液通过抗脂质过氧化,减轻血流变障碍从而减轻环孢霉素A引起的中毒性肾损伤。OBJECTIVE To explore the mechanism of antagonistic effects of Shenmai injection on nephrotoxicity induced by CsA in rats. METHODS The animals in 3 groups were respectively treated with olive oil, CsA, Shenmai injection plus CsA. After 4 weeks, blood was taken for measurements of serum creatinine, blood urea nitrogen and the hemorheology. The degree of renal injury was graded by the tubular pathological score. The renal tissue superoxide dismutase （SOD） and the content of malondialdehyde （MDA） in renal tissue were detected. RESULTS Severe renal damage was significant in cyclosporine-in- duced rats, and serum creatinine and blood urea nitrogen increased; Plasma viscosity, RBC aggregation and the content of fibrinogen increased obviously, while whole blood viscosity and hematoerit decreased significantly, these changes were con- spicuous in those rats treated with Shenmai injection. CONCLUSION The findings indicated that the hemorheological changes play important roles in renal injury induced by cyclosporine A, and Shenmai injection could effectively attenuate the nephro- toxicity, which contributed to the better microcirculation.

关 键 词：环孢霉素 肾毒性 参麦注射液 氧化损伤 血液流变学 

分 类 号：R334.1[医药卫生—人体生理学]