5-氮杂胞苷诱导脐带间充质干细胞向成肌细胞分化研究  

Umbilical cord mesenchymal stem cell was induced into myoblast with 5-azacytidine

作　　者：蓝建发[1,2] 罗新[1] 宋泓[1] 石海燕[1] 郭红宇[2] 

机构地区：[1]暨南大学附属第一医院妇产科,广东广州510000 [2]兰州大学第二医院妇产科,甘肃兰州730030

出　　处：《中国实用妇科与产科杂志》2013年第6期450-453,共4页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：国家自然科学基金(81070459)

摘　　要：目的探讨人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,HuMSCs)向成肌细胞分化。方法贴壁法分离HuMSCs;流式细胞仪分析其表面抗原;透射电镜及原子力电镜观察细胞超微结构;5-氮杂胞苷诱导细胞向成肌细胞分化;逆转录聚合酶链式反应(RT-PCR)检测成肌细胞特异基因结蛋白(desmin)和重链肌球蛋白(myosin heavy chain,MHC)表达;蛋白质印迹法(Western Blot)检测特异蛋白desmin和MHC表达。结果贴壁法稳定从脐带分离出细胞;HuMSCs不表达CD31、CD34、CD45,强表达CD29、CD90、CD44、CD105,极低表达HLA-DR、CD40;5-氮杂胞苷诱导后干细胞表达成肌细胞特异基因及蛋白明显高于对照组。结论贴壁法分离HuMSCs可靠、纯度高;5-氮杂胞苷可诱导HuMSCs向成肌细胞分化。Objective Whether eould human umbilical cord mesenehymal stem cell （HuMSC） differentiate into myoblast. Methods HuMSCs being isolated by adherent culture, eheeking surface markers by flow eytometry, observation of cells uhrastructural with transmission eleetron microscopy and atomic force electron microscopy ,5-Aza inducting HuMSCs to myoblast differentiation, RT-PCR and Western Blot detecting myoblast specific genes and protein expression respectively . Results Stem cells could isolate from umbilical cord with adherent euhure. HuMSCs donl express CD31, CIM5, CD34, strongly express CD29, CD90, CD44, CD105, and extremely low express HLA-DR,CD40. Expression of myoblast special genes and proteins was significantly higher between experimental group and control group. Conclusion Ad- herent culture was a reliable and economic method. 5-Aza could induce HuMSCs to myoblast differentiation.

关 键 词：脐带间质干细胞 结蛋白 重链肌球蛋白 5-氮杂胞苷 

分 类 号：R714.56[医药卫生—妇产科学]