抗逆转录因子在百脉根中的功能分析  

Functional analysis of drought-resistant transcription factors PeDREB2a and HhERF2 in Lotus corniculatus

作　　者：陶飞[1,2] 马江涛[2] 唐益雄[2] 李京生 卢运明 徐秉良[1] 吴燕民[2] 

机构地区：[1]甘肃农业大学草业学院草业生态系统教育部重点实验室甘肃省草业工程实验室中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]中国农业科学院生物技术研究所,北京100081 [3]深圳市农科集团有限公司,广东深圳518040

出　　处：《草业学报》2013年第3期250-258,共9页Acta Prataculturae Sinica

基　　金："973"项目(2007CB108902-2)资助

摘　　要：为提高豆科牧草百脉根抗旱方面的能力,从沙生植物胡杨和铃铛刺中克隆了4个抗逆转录因子基因并进行功能分析,从中筛选了2个具有抗旱特性的基因PeDREB2a和HhERF2,构建了以磷酸甘露糖异构酶为筛选标记的单、双价植物表达载体,转化百脉根Leo品种,结果表明,转录因子基因明显改善了百脉根的抗旱特性,经过抗旱复水试验证明转基因百脉根成活率达到100%。Two drought-resistant genes （PeDREB2a and HhERF2） were identified through functional analysis of the four transcriptional factor genes cloned from Populus euphraticaand Halimodendron halodendron, respectively. PeDREB2a and HhERF2 were two factors which had positive effects in plants against drought stress. Based on this, a series of plant expression vectors either harboring PeDREB2a or HhERF2 were constructed with a non resistant marker gene and transferred into the Lotus genome using an Agrobacterium mediated method. Transgenie Lotus had strong tolerance to drought. This showed that transcription factors significantly 100% improved drought resistance of L. corniculatus, and the survival rate of transgenic L. corniculatus was in a drought recovery water test.

关 键 词：百脉根 转录因子 磷酸甘露糖异构酶(PMI) 抗旱 转基因 

分 类 号：S541.6[农业科学—作物学]