漳州水仙ZDS基因cDNA克隆及其表达分析  

Cloning and Expression of ZDS Gene from Narcissus tazetta var. chinensis

作　　者：钟娴[1,2] 江琳玉[2,3] 潘东明[1,2] 潘腾飞[1,2] 卞阿娜[1,4] 

机构地区：[1]福建农林大学园艺产品贮藏保鲜研究所,福建福州350002 [2]福建农林大学园艺学院,福建福州350002 [3]龙岩市园林管理局,福建龙岩364000 [4]漳州师范学院生物科学与技术系,福建漳州363000

出　　处：《亚热带植物科学》2013年第2期97-103,共7页Subtropical Plant Science

基　　金：国家科技支撑计划项目(No.2007BAD07B00)

摘　　要：采用RACE技术从漳州水仙‘金盏银台’Narcissus tazetta var.chinensis花器官总RNA中克隆ZDS的cDNA序列,用相关软件进行序列分析,并利用实时荧光定量技术检测ZDS基因在各水仙品种不同花器官的表达。序列分析表明,ZDS基因cDNA全长2189 bp,其中包含69 bp 5'非编码区,395 bp 3'非编码区,1725 bp编码区(编码574个氨基酸,分子量约63.6 kDa),命名为Ntzds(GenBank登录号:EU573238),其编码的氨基酸序列(ACB87206.1)与喇叭水仙(CAA12062.1)、葡萄(XP_002277348.21)和温州蜜柑(ABC33728.1)ZDS基因编码产物的相似性分别为97%、85%和83%。实时荧光定量PCR分析表明,Ntzds基因在漳州水仙‘金盏银台’、‘Minnow’、‘Fortissimo’中均有表达,且同一品种中副冠的表达量高于花瓣;随着花色的加深,其转录水平也逐渐趋高。The ZDS gene was cloned from total RNA of flower in Narcissus tazetta var. chinensis by RACE technology. The expression analysis ofZDS gene was predicted with some related software tools, and the expression of ZDS gene in different floral organ of different varieties was analysis with real-time PCR. The sequence analysis indicated that the length of ZDS is 2189 bp, it contains an ORF encoding 499 amino acid residues（Mw=63.6 kDa）, as well as 5＇ non-coding region with 69 bp and 3＇ non-coding region with 395 bp, named as Ntzds （GenBank accession number EU573238）. Compared with ZDSs of other plants, Ntzds amino acid sequence has 97%, 85%, 83% identity with that of Narcissus pseudonarcissus, Vitis vinifera and Citrus unshiu respectively. Expressions of Ntzds in petals and corona from N. tazetta var. chinensis, ＇Minnow＇, ＇Fortissimo＇ were investigated by real-time qPCR. The results showed that Ntzds gene expression was different in three varieties. The expression level in corona was higher than in petals in same variety; we concluded that the transcription level of Ntzds was developing with the deepening of flower color.

关 键 词：漳州水仙'金盏银台’ ζ-胡萝卜素脱氢酶 基因克隆 基因表达 

分 类 号：S682.21[农业科学—观赏园艺] Q943.2[农业科学—园艺学]