环糊精-胶束毛细管电动色谱法检测土壤中联苯菊酯  

Determination of bifenthrin in soil by CD-MEKC

作　　者：宋旸[1] 陶玉贵[1] 曹宁[1] 金成[1] 孙忆晨[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000

出　　处：《安徽工程大学学报》2013年第2期11-13,共3页Journal of Anhui Polytechnic University

基　　金：安徽省高校省级自然科学研究重点基金资助项目(KJ2012A034)

摘　　要：建立了环糊精-胶束毛细管电动色谱(CD-MEKC)测定土壤中联苯菊酯的方法.考察了检测波长、运行缓冲体系、缓冲液浓度、缓冲液pH值、β-CD、SDS浓度和分离电压对联苯菊酯测定的影响.结果以70mmol/L硼酸(pH 8.0,含20mmol/Lβ-CD和70mmol/L SDS)作为缓冲溶液测定条件最佳,联苯菊酯能在19min内被检出,线性范围为15.625～500mg/L,线性相关系数为0.996 6,检出限(以信噪比(S/N)为3计)为0.176mg/L,加标回收率为97.2%～101.2%.精密度试验结果良好.该方法简便、快速、灵敏,可用于土壤中联苯菊酯含量的测定.A method for bifenthrin determination in soil based on micellar electrokinetic capillary chromatography was developed. The assay conditions including inspection wavelength, buffer types, buffer concentration, buffer pH value,β-CD, the concentration of sodium dodecyl sulfate（SDS） and the separation voltage were optimized. Under the optimized conditions（70 mmol/L boric acid buffer（pH 8. 0, containing 20 mmol/Lβ-CD, 70 mmol/L SDS）, the bifenthrin can be separated within 19 rain, with the linearworking range of 15. 625～500 mg/L （r^2 = 0. 996 6） and a limit of detection （S/N=3） of 0. 176 mg/L. It has good recoveries （97.2～101.2M） and the relative standard deviations is lower than 3 %. The method is simple, rapid, sensitive, highly reproducible and can be successfully applied in the determination of bifenthrin in soil.

关 键 词：环糊精-胶束毛细管电动色谱(CD-MEKC) 联苯菊酯 检测 土壤 

分 类 号：O657.8[理学—分析化学]