苦参碱对大鼠脑胶质瘤肿瘤干细胞增殖的影响  

Effect of sophocarpidine on proliferation of glioma tumor stem cell in rats

作　　者：杨赣军[1] 张小强[1] 孙弋[1] 

机构地区：[1]井冈山大学医学院解剖教研室,江西吉安343000

出　　处：《甘肃医药》2013年第6期417-419,共3页Gansu Medical Journal

摘　　要：目的:检测苦参碱对C6胶质瘤肿瘤干细胞增殖的抑制作用,从而探讨其作用机制。方法:体外培养脑C6胶质瘤肿瘤干细胞,实验分为人参皂苷Rg3组、阴性对照组及阳性对照组。采用MTT法测定苦参碱对细胞的吸光值并计算抑制率;运用BrdU免疫组织化学方法来检测苦参碱对C6胶质瘤肿瘤干细胞增殖的影响。结果:MTT结果表明细胞抑制在苦参碱作用后明显,而免疫组化染色显示苦参碱可有效抑制C6胶质瘤肿瘤干细胞的增殖。结论:苦参碱能显著抑制脑胶质瘤肿瘤干细胞的增殖,这可能是其参与抗肿瘤的机制之一。Objective： To detecte the inhibition role of sophoearpidine on glioma tumor stem cell in order to probe its mechanism of action. Methods：C6 glioma tumor stem ceil were cultured in vitro in which the study was divided into the sophoearpidine group,the negative control group,and the positive control group. The cell light absorption value was detected by MTT methods and the inhibition ratio was calculated,and the effect of sophocarpidine on the proliferation of C6 glioma tumor stem cell was detected by BrdU immunohistochemistry. Results：The ceil light absorption value was obviously decreased in the sophocarpidine group by the MTY detecting. The immumohistoehemical staining showed that sophocarpidine obviously inhibited the proliferation of C6 glioma tumor stem ceil.Condusion：Sophoearpidine obviously inhibited the proliferation of C6 glioma tumor stem cell, which may one of the mechanism actions for sophoearpidine anti-tumor.

关 键 词：苦参碱 胶质瘤 肿瘤干细胞 BRDU 

分 类 号：R339.141[医药卫生—人体生理学]