低强度激光照射对离体家兔红细胞膜磷脂成分及电泳率的影响  

The influence of low Power Laser Irradiation on phospholipid composition ofmembrane and electrophoresis rate of red blood cell in rats

作　　者：徐承水[1] 王文房[2] 赵云峰[3] 

机构地区：[1]曲阜师范大学教育科学学院,山东曲阜273165 [2]临沂大学生命科学学院,山东临沂276005 [3]曲阜师范大学生命科学学院,山东曲阜273165

出　　处：《激光杂志》2013年第3期37-38,共2页Laser Journal

基　　金：山东省实验改革研究课题(2010-431);曲阜师范大学研究课题(XJ0675)资助

摘　　要：目的:探讨低强度激光对离体家兔红细胞膜磷脂成分及电泳率的影响。方法:用低强度激光照射家兔红细胞和血小板,以薄层色谱扫描法和显微(细胞)电泳法测定。结果:实验组红细胞膜磷脂成分中磷脂酰胆碱显著增多(p<0.01),而鞘磷脂类、磷脂酰乙醇胺都程度不同的减少(p<0.05),特别是磷脂酰丝氨酸含量、鞘磷脂类/磷脂酰胆碱及磷脂酰丝氨酸/磷脂酰胆碱比值显著减小(p<0.01)。血小板及红细胞的电泳率显著高于对照组。结论低强度激光照射可改变离体家兔红细胞膜磷脂成分和电泳率。AIM In this paper,we discussed the influence of low power laser to phospholipid composition of membrane of red blood cell and electrophoresis rate of rats.Methods:Adopting red blood cell and blood platelet of rats,irradiated with He-Ne laser,determining by lamina chromatogram scanning and cell electrophoresis with microscope.Results:In experimental groups,PC(phosphatidy choline) of phospholipid composition of membrane of red blood cell was diminished evidently,and SM(sphingomyelin)、PE(phosphatidyl ethanolamines) was diminished incoordinately,especially SM/PC and PS/PC was diminished evidently.Electrophoresis rate of the blood platelet and red blood cell was obviously higher than that of comparisonal groups.CONCLUSIONS low power laser irradiated can change red blood cell in rats's membrane lipids and electrophoresis rate.

关 键 词：低强度激光照射 红细胞膜 膜脂 电泳率 

分 类 号：R602[医药卫生—外科学] TN248.1[医药卫生—临床医学]