β-榄香烯对人胃癌SGC-7901细胞增殖抑制作用及对Bax和Bcl-2蛋白表达的影响  

Inhibitory Effect of β-elemene on Human Gastric Cancer SGC-7901 Cells and its Influence on Expression of Bcl-2 and Bax Proteins

作　　者：韩莹[1] 张玲[1] 吴璠[1] 

机构地区：[1]沈阳医学院基础医学院病理教研室,辽宁沈阳110034

出　　处：《实用预防医学》2013年第6期748-750,共3页Practical Preventive Medicine

摘　　要：目的探讨β-榄香烯对人胃癌SGC-7901细胞增殖抑制作用及对Bax和Bcl-2蛋白表达的影响,以进一步了解其作用机制。方法用含有10%小牛血清的RPMI1640培养基在37℃下,体积分数5%的二氧化碳培养箱中培养人胃癌SGC-7901细胞,以三氧化二砷为阳性对照药物,采用MTT法对比β-榄香烯与三氧化二砷在不同浓度下对细胞增殖的影响,用Western blot法检测Bax和Bcl-2蛋白的表达情况。结果β-榄香烯和三氧化二砷在浓度为20、40以及80μmol/L时,对人胃癌SGC-7901细胞增殖有显著的抑制作用(P<0.05);而β-榄香烯在浓度为40、80μmol/L时,对人胃癌SGC-7901细胞增殖的抑制作用较三氧化二砷更加明显(P<0.05)。随着β-榄香烯的浓度逐渐升高,人胃癌SGC-7901中促进细胞凋亡的Bax蛋白的表达上调,而抑制细胞凋亡的Bcl-2蛋白的表达下调。结论β-榄香烯能够有效抑制人胃癌SGC-7901细胞的增殖,并且能够通过调控Bax和Bcl-2蛋白表达,从而达到诱导肿瘤细胞凋亡的目的。Objective To explore the inhibitory effect of β-elemene on human gastric cancer SGC-7901 cells in vitro and its impact on expression of Bcl-2 and Bax proteins so as to further study its mechanism.Methods The effect of β-elemene on human gastric cancer SGC-7901 cells was observed with arsenous oxide as positive control.The inhibition of drugs on gastric cancer cells was evaluated with MTT assay.The levels of Bcl-2 and Bax proteins in gastric cancer cells treated with different concentrations of β-elemene were detected with Western Blot.Results MTT assay indicated a significant inhibitory effect of β-elemene on SGC-7901 cells with the concentrations of 20,40,and 80 μmol/L,respectively.β-elemene up-regulated the level of Bax protein,but down-regulated the level of Bcl-2 protein.Conclusion β-elemene has a significant inhibitory effect on human gastric cancer SGC-7901 cells by causing the imbalance of the ratio of Bax/Bcl-2.

关 键 词：Β-榄香烯 胃癌 BAX BCL-2 

分 类 号：R332[医药卫生—人体生理学]