妊娠相关血浆蛋白A重组抗原表位分析及抗体制备  

Study on epitopes of recombinant pregnancy-associated plasma protein A

作　　者：曾昭伟[1] 王学谦[2] 常艳敏[1] 蔡迪娅[1] 

机构地区：[1]天津市南开医院,天津300100 [2]天津市天津医院

出　　处：《山东医药》2013年第13期26-28,31,共4页Shandong Medical Journal

基　　金：天津市科技计划项目(08ZCGYSF01800)

摘　　要：目的探讨妊娠相关血浆蛋白A(PAPP-A)的抗原表位及抗PAPP-A抗体制备方法。方法把PAPP-A的CDS序列翻译成氨基酸序列,利用互联网分析蛋白的疏水性和信号肽区段,利用DNAstar软件分析二级结构、表面可及性、亲水性、抗原指数以及柔韧性,综合分析抗原表位。通过分子生物学技术制备重组PAPP-A,进而免疫新西兰家兔制备抗PAPP-A抗体。结果 PAPP-A抗原性较强,抗原优势表位位于23～94、1 110～1 131氨基酸区段;PAPP-A重组蛋白以及抗PAPP-A抗体制备成功。结论本研究方法建立了PAPP-A的高灵敏免疫检测体系,能进一步提高PAPP-A的临床应用价值。Objective To analyze epitopes of PAPP-A and prepare pregnancy-associated plasma protein A （PAPP-A） antibody. Methods CDS region of PAPP-A was translated into amino acid sequencing form; then hydrophobicity and sig- nal peptide of PAPP-A were analyzed by Intcrnet; the secondary structure, surface probability, hydrophilicity, antigen in- dex and flexible region were all analyzed by DNAstar; then the antigenic epitopes were analyzed by the above means. Re- combinant PAPP-A antigen was made by a series of molecular biology techniques and then it was injected into rabbits to make PAP-A antibody. Results Antigenicity of PAPP-A was strong, the region was located in 23-94, 1110-1131 region of the amino acid sequencing. The recombinant PAPP-A antigen and its antibody were managed to prepare. Conclusion The high sensitive immunoassay of PAPP-A was established in this study, which can furthur improve the clinical application val- ue of PAPP-A.

关 键 词：妊娠相关血浆蛋白A 二级结构 抗原表位 抗体 

分 类 号：R714.14[医药卫生—妇产科学]