超高压辅助浊点萃取法提取富集莲子心总生物碱  

Extraction and enrichment of total alkaloids of Plumula Nelumbinis by ultrahigh pressure-assisted micellar extraction combined with cloud-point extraction

作　　者：朱荣华[1,2] 王晓[2] 刘建华[2] 张树芹[1] 刘峰[2] 

机构地区：[1]山东农业大学化学与材料科学学院,山东泰安271018 [2]山东省分析测试中心,山东济南250014

出　　处：《山东科学》2013年第3期15-20,25,共7页Shandong Science

基　　金：山东省科技发展计划(2011SJGZ19);山东省分析测试中心科技发展计划项目

摘　　要：通过表面活性剂类型、浓度、提取压力、时间、料液比等单因素实验确定了超高压辅助胶束提取的工艺条件,并通过加入NaCl和加热引发体系浊点,诱导双水相分离,实现了莲子心生物碱的富集。结果表明,最佳提取工艺为质量分数5%的OP-10为溶剂,提取压力100 MPa,提取时间1 min,料液比1:20(g/mL),莲子心生物碱的提取率可达到103.6 mg/g。提取液中加入NaCl使其质量分数达到15%,在50℃条件下平衡10 min,两相分离,离心后莲子心生物碱的萃取回收率为70.73%,浓缩系数为8.6。此方法快速高效、环境友好。We determined the technical conditions of ultrahigh pressure-assisted micellar extraction by such single-element experiment as surface active agent type,concentration,extraction pressure,time and the ratio of solid and liquid to construct the extraction and enrichment process of total alkaloids of Plumula Nelumbinis.We implemented the enrichment of the alkaloids of Plumula nelumbinis by NaCl addition,cloud point extraction and double water phase separation.The optimal extraction conditions are 5%（w/w） OP-10 solvent,extraction pressure of 100 MPa,extraction time of 1 min and solid/liquid ratio of 1：20（g/mL）.The optimal extraction yield of total alkaloids is 103.6 mg/g.Its recovery rate is 70.73% and the pre-concentration factor（CF） is 8.6 under the conditions of 15% NaCl addition,stability of 10 min at 50 ℃ and two-phase separation.Results show that the extraction process is rapid and benefit for environment.

关 键 词：莲子心 生物碱 超高压辅助胶束提取 浊点萃取 

分 类 号：R284.2[医药卫生—中药学]