鹤顶兰嵌合体分离研究初探  

Separation of Phaius tankervilleae Chimera

作　　者：李豆豆[1] 唐燕琼[1] 尹俊梅[2] 王毅[3] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带作物品种资源研究所,海南儋州571737 [3]海南省琼州学院生物科学与技术学院,海南三亚572022

出　　处：《热带农业科学》2013年第5期43-45,共3页Chinese Journal of Tropical Agriculture

基　　金：教育部热带作物新品种选育工程中心与作物学重点学科联合资助项目(No.lhxm-2012-7);2013年海南省自然科学基金项目(No.313032);公益性行业(农业)科研专项经费项目(No.201203071);三亚市院校专项资金项目(No.YD09036)

摘　　要：以秋水仙素诱导获得的鹤顶兰嵌合体无菌苗的假鳞茎、茎尖及除根苗为外植体,分别接种于花宝一号的固体、半固体及固液双层培养基上进行培养,通过诱导类原球茎的方法对嵌合体进行分离。结果表明,鹤顶兰嵌合体分离最佳方法为将除根苗插入花宝一号+100.0 ml/L椰子水+2.0 g/L活性炭+20.0 g/L蔗糖+2.0 mg/LBA+0.2 mg/LNAA的半固体培养基上,诱导出PLB进而分化成苗,分离得到纯合四倍体。In order to obtain pure teraploid of Phaius tankervilleae, improve polyploidy induction efficiency. Through inducting protocorm like body for separation of chimera, the research used the pseudobulb, root segment, shoot apex as explants cultured with Hyponex No.l, solid, semi-solid and liquid-solid double-layer medium. The results showed that the best method of inducting protocorm iike body was inserting the resection of the plant root to semi-solid Hyponex No.1 ＋ 100 mYL CW ＋ 2 g/L AC ＋ 20 g/L sucrose ＋ 2.0 mg/L BA ＋ 0.2 mg/LNAA. Two pure teraploid were obtained from this. The method can be used to separate Phaius tankerviUeae chimera.

关 键 词：鹤顶兰 嵌合体 组织培养 四倍体 

分 类 号：S682.31[农业科学—观赏园艺]