基于杜仲转录组序列的SSR分子标记的开发  

Development of SSR Molecular Markers Based on Transcriptome Sequencing of Eucommia ulmoides

作　　者：黄海燕[1] 杜红岩[1] 乌云塔娜[1] 刘攀峰[1] 

机构地区：[1]中国林业科学研究院经济林研究开发中心国家林业局杜仲工程技术研究中心,郑州450003

出　　处：《林业科学》2013年第5期176-181,共6页Scientia Silvae Sinicae

基　　金：国家林业公益性行业科研专项(201004029);国家"十二五"科技支撑计划(2012BAD21B0502)

摘　　要：简单重复序列（simple sequence repeat,SSR）,又称微卫星,是广泛存在于真核和原核生物基因组中的1～6个核苷酸串联重复单元,研究发现基因组中平均每50kb就有1个SSR（Morgante et al.,1993;Kalia et al.,2011）,SSR标记主要包括基因组SSR和表达序列标签SSR（EST-SSR）。To study the genetic diversity of Eucommia ulmoides without information of the whole genome, the SSR primers were designed based on the transcriptome sequencing data （unpublished） from leaves and fruits of E. ulmoides. The SSR loci were analyzed using microsatellite locus scan tool SSRIT to analyze 49 610 sequences, and we screened out 1 442 SSR loci which distributed in 1 334 sequences, accounting for 2.9% of the transcriptome sequences. The dinucleotide repeat is the most abundant repeat type, accounting for 69.90% of the total number of SSRs. We observed 150 kinds of repeating units and found that the highest frequency is AG/TC, accounting for 32.73% of the total number of SSRs. Additionally, we found a small amount of CG repeats. A total 85 pairs of primers were designed and synthesized, and the primers were verified by using 8 different excellent clones and among them 50 pairs of primers were able to amplify products, of which 20 pairs of primers were polymorphic. This study had an important application value to analyze genetic diversity of E. ulmoides by using SSR molecular markers.

关 键 词：杜仲 转录组 SSR 引物 

分 类 号：S718.46[农业科学—林学]