亚氨基二乙腈的高效液相色谱法测定  

Determination of Iminodiacetonitrile by High Performance Liquid Chromatography

作　　者：邱露[1] 冉艳琼 

机构地区：[1]成都大学生物产业学院,四川成都610106 [2]四川省天然气化工研究院新产品研究所,四川成都610212

出　　处：《化学与生物工程》2013年第6期91-94,共4页Chemistry & Bioengineering

基　　金：国家863计划资助项目(2009AA032901)

摘　　要：建立了利用高效液相色谱分析亚氨基二乙腈的方法。色谱条件为:Agilent TC-C18(2)色谱柱(4.6mm×250mm,5μm),以水-乙腈缓冲溶液(体积比80∶20,pH=5.5)为流动相,流速为1.0mL.min-1,紫外检测器波长为205nm,色谱柱温度为35℃。结果表明,在该色谱条件下亚氨基二乙腈及其聚合物亚甲基二亚氨基二乙腈得到了较好的分离,亚氨基二乙腈的浓度在0.64～9.60g·L-1范围内与峰面积呈良好的线性关系,相关系数为0.99907,精密度RSD为0.37%,回收率为99.35%～101.61%。该方法比传统的酸碱滴定法更准确、可靠。A determination method for IDAN by HPLC was established. Chromatographic conditions. Agi- lent TC-C18 （2） （4.6mm×250mm,5μm） column, mobile phase was water-acetonitrile buffer （80：20, pH= 5.5） ,flow rate was 1.0 mL·min^-1 ,UV detection wavelength was 205 nm,chromatographic column tempera- ture was 35℃. Results showed that u centration of IDAN had a good linear coefficient was 0. 99907, the RSD of method was more reliable and accurat nder above conditions,IDAN and MBIDAN were separated well. The con- correlation with its peak area within a range of 0.64-9.60 g ·L^-1 ,the precision was 0. 37%, the recovery rate was 99.35%- 101. 61%. This e than the traditional acid-alkali titration method.

关 键 词：亚氨基二乙腈 亚甲基二亚氨基二乙腈 高效液相色谱 测定 

分 类 号：O657.72[理学—分析化学] TQ226.61[理学—化学]