解毒活血方对AS易损斑块兔模型易损斑块及CAM-1、TNF-α表达的影响  

Effect of Jieduhuoxue Decoction on Vulnerable Plaque and CAM-1、TNF-α Expression in Vulnerable Plaque Rabbits′Models

作　　者：吴辉[1] 卿立金[1] 吴伟[1] 李文晞[1] 李荣[1] 刘勇[2] 刘煜德[1] 黄衍寿[1] 

机构地区：[1]广州中医药大学第一附属医院,广东广州510405 [2]广州中医药大学,广东广州510405

出　　处：《中国中医急症》2013年第6期877-879,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：广东省科技计划项目(20090315)

摘　　要：目的探讨解毒活血中药对易损斑块病理及炎症因子人细胞间黏附分子-1(ICAM-1)、肿瘤坏死因子-α(TNF-α)表达的影响。方法通过球囊损伤主动脉内膜联合高脂饲料建立实验性动脉粥样硬化易损斑块动物模型,造模兔随机分低剂量中药组、高剂量中药组、辛伐他汀组、模型对照组,分别予以低剂量解毒活血方、高剂量解毒活血方、辛伐他汀、生理盐水灌胃;另设正常对照组以生理盐水灌胃,疗程10周。观察主动脉HE染色病理改变、斑块ICAM-1、TNF-α免疫组化及ELISA检测血清ICAM-1、TNF-α表达改变。结果模型对照组呈现易损斑块特点,斑块内ICAM-1、TNF-α免疫组化正常对照组为阴性,模型对照组呈强阳性表达,高剂量中药组表达显著降低;低剂量中药组呈阳性表达,辛伐他汀组TNF-α呈弱阳性表达,高剂量中药组、辛伐他汀组血清ICAM-1、TNF-α水平降低,与模型对照组比较有明显差异。结论解毒活血方对实验性兔动脉粥样硬化易损斑块的形成具有预防作用,能降低易损斑块内及血清TNF-α、ICAM-1表达,特别是抑制易损斑块底部和肩部的炎症。Objective： To investigate the effect of Jieduhuoxue Decoction on vulnerable plaque and CAM-1 and TNF-α expression in vulnerable plaque rabbits′ model.Methods： The forty vulnerable plaque models were eastablished by injuring the rabbits′ abdominal-thoracic aorta intima with the balloon-injured angioplasty through femoral artery at the second week,then followed with high cholesterol feeding beginning at the third week.These forty model rabbits were randomly divided into four groups such as the model control group,the simvastatin treating group,the small-dose Jieduhuoxue Decoction group,the large-dose Jieduhuoxue Decoction group.At the third week,first of drug interventions were added during the process.And there was a normal control group those were ten healthy male New Zealand white rabbits fed with standard feed.After administered for 10 weeks,the concentrations including ICAM-1 and TNF-α in the serum were examined by ELISA.Some indices were observed and detected including the morphology of the atherosclerotic vas,the expression of ICAM-l and TNF-α in the artery with immunohistochemistry.Results： The vulnerable plaque animal model was successfully established.The aortic plaques of large-dose Jieduhuoxue Decoction group showed characteristics of stable plaque.Immunohistochemistry results found that ICAM-1 and TNF-α plaque was negative in the control group.It showed strong positive expression in model control group which was significantly decreased in the large-dose Jieduhuoxue Decoction group.This index in the small-dose Jieduhuoxue Decoction group was positive.The simvastatin group TNF-α showed weak positive expression.From the ELISA results,the TNF-α levels were reduced in the large-dose Jieduhuoxue Decoction group and the simvastatin group.Meanwhile,the serum ICAM-1 and TNF-α levels were reduced ompared with the model group.Conclusion： Jieduhuoxue Decoction could prevent atherosclerosis vulnerable plaque by lowering the expression of ICAM-1 and TNF-a in vulnerable plaque rabbits＇model.The o

关 键 词：解毒活血 易损斑块 ICAM-1 TNF-Α 

分 类 号：R285.5[医药卫生—中药学]