针刺水沟、风府穴对急性大鼠脊髓损伤后前炎性因子表达的影响  

Effects of Acupuncture at Shuigou (GV26) and Fengfu (GV16) on the Expression of Pro-inflammation Cytokine after Spinal Cord Injury

作　　者：邹恩苗[1] 王赛[1] 李江筎[1] 蒋松鹤[1] 

机构地区：[1]温州医学院附属第二医院,浙江温州325027

出　　处：《中国中医急症》2013年第6期883-886,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：浙江省自然科学基金课题(LY12H27002);浙江省温州市科技局基金项目(Y20110069)

摘　　要：目的观察针刺水沟,风府穴对大鼠急性脊髓损伤(SCI)后前炎性因子表达及神经细胞凋亡的影响。方法雄性SD大鼠36只,随机分为假手术组、模型组、针刺组,采用实时荧光定量PCR法检测前炎性因子包括白介素-6(IL-6),白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)mRNA的表达情况;用原位末端标记法(TUNEL)检测SCI后神经细胞凋亡情况,用HE及Nissle染色观察组织细胞形态变化。结果 SCI后8 h,模型组与假手术组比较受损伤的脊髓组织中模型组中前炎性因子mRNA表达水平显著升高,且TUNEL阳性细胞数增多,针刺组大鼠受损伤的脊髓组织中前炎性因子mRNA表达水平与TUNEL阳性细胞数较模型组均减少。结论针刺水沟风府穴可降低大鼠急性SCI后脊髓组织中前炎性因子的表达及神经细胞凋亡的发生。Objective：To investigate the effect of acupuncture at Shuigou（GV26） and Fengfu（GV16） on neuronal apoptosis and the expression of pro-inflammation cytokine after spinal cord injury in rats.Methods：36 SD rats were divided into 3 groups： sham-operated group,model group,acupuncture group.The expression of TNFa,IL-1β and IL-6 mRNA were detected by quantitative Real Time-PCR（qRT-PCR）.Apoptosis neuron was marked with terminal deoxynucleotidyl transferase-midiated deoxyuredine triphosphate-biotin nick end labeling（TUNEL） staining.Organizational changes in cell morphology were observed by HE and Nissle dyeing.Results： Comparing with sham-operated group,the expression of mRNA in model group was markedly increased in the injured spinal cord at 8h after injury,and there were more TUNEL-positive cells in model group than in shamoperated group.Compared with control group,acupuncture significantly reduced the expression of TNF-α,IL-1β and IL-6 mRNA,and decreased the number of TUNEL-positive cells.Conclusion：Acupuncture may inhibit the expression of expression of pro-inflammation cytokine and reduce neuronal cell apoptosis after SCI.

关 键 词：脊髓损伤 针刺 前炎性因子 凋亡 

分 类 号：R245[医药卫生—针灸推拿学]