NaCl胁迫对桑树叶片DNA变异的影响  

Effects of NaCl on DNA damage in leaves of Morus alba

作　　者：张薇[1] 刘岩[1] 计东风[1] 沈卫锋[1] 林天宝[1] 朱燕[1] 孟智启[1] 吕志强[1] 

机构地区：[1]浙江省农业科学院蚕桑研究所,浙江杭州310021

出　　处：《浙江农业学报》2013年第3期435-438,共4页Acta Agriculturae Zhejiangensis

基　　金：浙江省蚕桑农业新品种选育重大科技专项(2012C12910)

摘　　要：利用RAPD标记技术检测桑树叶片的DNA损伤程度可以作为衡量桑树耐盐性的指标。分别用0.2%和0.4%NaCl溶液胁迫桑苗8周后对桑树叶片的基因组DNA进行RAPD分析。16条RAPD引物均为多态性片段,共扩增出59条带,平均每个引物扩增出3.69个条带。用Nysyspc2.1软件计算出不同处理组桑树叶片DNA的遗传相似系数为0.3662～0.5476。树状聚类图显示处理组桑树叶片DNA聚为一类,对照组桑树叶片DNA自成一类,说明盐胁迫浓度与桑叶DNA损伤呈正相关,DNA损伤的变异程度可以作为桑树耐盐性品种选育的一个有效参考指标。DNA damage of mulberry （Morus alba） leaf detected by RAPD marker technology could be used as a ref- erence for evaluating the salt tolerance of mulberry. After treated by 0. 2% and 0. 4% NaC1 solution for 8 weeks, mulberry genomic DNA was isolated for RAPD assay. After using 16 RAPD primers to amplify the genomic DNAs of three samples, 59 clearly distinguishable bands were obtained, 3.69 bands per primer on average. Calculation with Ntsyspc 2. 1 software displayed that the genetic similarity coefficients between DNAs from different treatments were in the range of 0. 3662 - 0. 5476. The result of dendrogram showed that the samples treated with 0.2% and 0. 4% NaCl solution were clustered into one group, and the normal sample was clustered into one group. These results showed that NaCl concentration has positive correlation with DNA damage of mulberry leaf, and the degree of DNA damage in mulberry leaf can be an effective reference of selecting salt-enduring mulberry varieties.

关 键 词：盐胁迫 桑树 DNA损伤 随机扩增多态性DNA 

分 类 号：S888[农业科学—特种经济动物饲养]