水稻类病变突变体spl5胚性悬浮细胞系的构建  

Establishment of embryogenic suspension cell line of rice lesion mimic mutant spl5

作　　者：金杨[1] 王月[1] 于诗莹[1] 陈析丰[1] 马伯军[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《浙江农业学报》2013年第3期456-460,共5页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金项目(31171519;31101130);浙江省自然科学基金项目(Y3110234;Y3110230);浙江省重点科技创新团队(2010R50024);浙江省公益性技术应用研究计划项目(2011C22005)

摘　　要：类病变突变体(lesion mimic mutant)是一类研究细胞死亡与抗病机制的有效工具。试验以水稻类病变突变体spl5为材料,构建了spl5突变体的胚性悬浮细胞系。结果表明诱导spl5突变体愈伤组织的最佳2,4-D浓度为2 mg·L-1,疏松的Ⅱ型胚性愈伤组织经悬浮继代培养3～4个月后可获得理想的胚性悬浮细胞系,FDA-PI染色结果表明该细胞悬浮体系活力高。水稻类病变突变体spl5胚性悬浮细胞系的建立可为进一步研究spl5突变体细胞死亡及其与病原菌互作的机制奠定基础。Lesion mimic mutant is a powerful tool to study the mechanisms of cell death and disease resistance. This study established a suspension-cell line of spl5, a rice lesion mimic mutant. For spl5 mutant, the optimum concentra- tion of 2,4-D was 2 rag. L -1 in callus induction and the ideal embryogenic suspension-cell line, from osteoporosis type Ⅱ embryogenic callus, was achieved after cultured 3-4 months in suspension medium. After FDA-PI staining, the suspension-cell line of spl5 mutant showed high viability, and could be used in the further study on the mechanism of cell death and interaction with pathogen in spl5 mutant.

关 键 词：水稻 类病变突变体 spl5 悬浮细胞系 

分 类 号：S511[农业科学—作物学]