牛日本血吸虫病抗体检测试纸条的研制及初步评价  

Preparation and preliminary evaluation of the rapid diagnostic strip to detect the bovine antibody against Schistosoma japonicum

作　　者：卢福庄[1] 俞国乔 周煜 阳爱国[4] 付媛[1] 顾昀[1] 董国栋[5] 

机构地区：[1]浙江省农业科学院畜牧兽医研究所,浙江杭州310021 [2]浙江省畜牧兽医局,浙江杭州310020 [3]湖北省动物疫病预防控制中心,湖北武汉430067 [4]四川省动物疫病预防控制中心,四川成都640041 [5]云南省动物疫病预防控制中心,云南昆明650051

出　　处：《浙江农业学报》2013年第3期467-474,共8页Acta Agriculturae Zhejiangensis

基　　金：浙江省优选主题重点农业项目(2009C12057)

摘　　要：为了建立一种检测牛日本血吸虫病的快速诊断技术,试验以乳胶微球标记的兔抗牛IgG抗体作为免疫探针,以血吸虫可溶性抗原为检测线,羊抗兔IgG为质控线,研制了牛日本血吸虫病抗体检测试纸条。对该试纸条进行了效价、敏感性、特异性、非特异性、保存期、批内批间可重复性及符合率等各项试验,结果表明试纸条的效价为1∶2;对于实验感染日本血吸虫28 d和35～56 d的病牛血清,试纸条的敏感性分别为20%(2/10—检出阳性份数/检测数)和100%(30/30);用于检测健康牛血清的特异性为100%(20/20—检出阴性份数/检测数),检测东毕吸虫、肝片吸虫、锥虫病牛血清,未见交叉反应现象;对于粪孵确诊为血吸虫病阴、阳性的牛血清,试纸条与粪孵法的阴阳性符合率均为100%;与IHA阴阳性符合率也均为100%;试纸条批内批间可重复性好,2～8℃保存的试纸条有效期达15个月。用试纸条检测实验感染血吸虫牛治疗前后体内不同时期血清抗体的水平,发现牛感染血吸虫后第5周的血清均可检测出血吸虫抗体,在治疗后第16～24周血吸虫抗体消失。In order to establish a rapid method to diagnose bovine schistosomiasis, a diagnostic strip to detect the bo- vine antibody against Schistosoma japonica was trial-produced based on using rabbit anti-bovine IgG labelled with red latex as an immunoprobe, using soluble egg antigen of Schistosoma japomicum and goat anti-rabbit IgG as a test line and control line, respectively. A series of studies were conducted to evaluate the application values of the strip. The results showed that the titer of the strip was 1：2. The sensitivities to detect the 40 sera from cattle which had been in- fected with Schistosoma japonicum cercariae for 28 days and 35-49 days were 20% （2/10 -detected positive reaction simple number/detected simple number） and 100% （30/30）, respectively. The specificity to examine the health bovine sera was 100% （20/20 -detected negative reaction simple number/detected simple number） , and no cross reaction was found when the sera from Orientobilharziasis, Fasciolosis and Trypanosomaisis cattle were detected. To check up on the positive sera from schistosomiasis cattle which had been confirmed by stool hatching test for exami- ning Schistosoma japonicum miracidium, the negative and positive coincidence rates between the strip assay to stool hatching test was all 100% , and the negative and positive coincidence rates between the strip assay to IHA was all 100% , too. The strip assay had excellent repeatability firmed by intra- and inter-batch assay. The validity duration of the strip which was stored in 2-8℃ was 15 months. When the strips were detected the sera prepared from cattle in different periods after infecting Schistosoma japonicum cercariae and curing with praziquantel, it was found that the schistosomiasis antibodies were early detected in all bovine sera on 35 d after infecting cercariae, and the schistoso- miasis antibodies disappeared on and after 112-168 d after being cured.

关 键 词：血吸虫病 抗体 检测试纸条 牛 

分 类 号：S854.43[农业科学—临床兽医学] S855.9[农业科学—兽医学]