环境温度变化致大鼠小肠黏膜机械屏障损伤的研究  

Ambient Temperature Changes Induce Small Intestinal Mucosal Mechanical Barrier Dysfunction in Rats

作　　者：吴芳庚[1,2] 曾慧红[3] 傅爱香[1] 庄德葆[1] 胡国柱[4] 朱清仙[3] 

机构地区：[1]南昌大学研究生院医学部,2008级南昌330006 [2]江西省肿瘤医院病理科,南昌330029 [3]南昌大学基础医学院组织胚胎学教研室,南昌330006 [4]江西省人民医院中心实验室,南昌330006

出　　处：《南昌大学学报（医学版）》2013年第2期14-16,34,F0003,共5页Journal of Nanchang University：Medical Sciences

基　　金：国家"十一五"科技支撑计划(2008Bai68b01);江西省自然科学基金(2007GQY0997);江西省卫生厅科技计划(20112012);2009年江西省研究生创新专项资金(YC09A011)

摘　　要：目的通过观察在环境温度发生骤然变化时大鼠小肠黏膜的病理损伤,探讨小肠黏膜机械屏障受环境温度影响的变化规律。方法将168只大鼠按随机数字表法均分为正常温度组(20℃、22只)、温度骤变组[骤降温至4℃组(22只)、骤降温至-12℃组(22只)、骤升温至37℃组(22只)],各组均观察7个时间点,即1、4、8、12、24、48和168h,每个时间点6只大鼠。常规HE染色观察十二指肠、空肠及回肠的组织病理学变化。结果温度骤变组大鼠肠黏膜组织均出现不同程度的损伤,出现绒毛水肿,毛细血管扩张充血、出血,炎细胞浸润等病理变化。在骤升温至37℃组损伤最严重,各组出现损伤最严重的时间不同。结论环境温度骤升或骤降都可引起小肠黏膜机械屏障损伤;环境温度骤升使大鼠肠黏膜的机械屏障损伤更严重。Objective To investigate the influence of ambient temperature changes on small in testinal mucosal mechanical barrier through observating the relationship between ambient temper- ature changes and small intestinal mucosal injury in rats. Methods A total of 168 rats were ran domly divided into four groups： normal temperature （20 ℃ group）,sudden drop in temperature to 4 ℃ （4 ℃ group）,sudden drop in temperature to -12 ℃ （-12 ℃ group） and sudden rise in tem- perature to 37 ℃ （37 ℃ group）,with 22 rats in each group. Six rats in each group were observed at 1,4,8,12,24,48 and 168 hours, respectively. Histopathological changes in the duodenum, jeju- hum and ileum were observed by HE staining. Results Rats undergoing ambient temperature changes had different degrees of damage in intestinal mucosa, including villous edema, capillary dilatation, congestion and bleeding, and inflammatory cell infiltration. The most obvious damage was found in 37 ℃ group. In addition,time point for the most obvious damage varied among dif- ferent groups. Conclusions Ambient temperature changes,especially sudden rise in temperature, can result in small intestinal mucosal mechanical barrier dysfunction.

关 键 词：环境温度变化 小肠 病理损伤 动物 实验 大鼠 

分 类 号：R332[医药卫生—人体生理学] R361.2[医药卫生—基础医学]