7种植物多糖对小鼠卵清蛋白免疫反应的佐剂活性(英文)  

Adjuvant activities of seven natural polysaccharides on im mune responses to ovalbum in in mice

作　　者：董娜[1,2] 张贵强[3] 贾培媛[2] 武军华[2] 李帅[2] 单俊杰[2] 王玉霞[2] 

机构地区：[1]中国人民解放军总医院,北京100853 [2]军事医学科学院毒物药物研究所,北京100850 [3]贵州大学生命科学院,贵州贵阳550025

出　　处：《中国药理学与毒理学杂志》2013年第3期307-314,共8页Chinese Journal of Pharmacology and Toxicology

基　　金：国家重点基础研究发展计划(973计划)(2010CB933904);国家十二五科技支撑项目(2011BAD26B02)~~

摘　　要：目的寻找和筛选具有较好佐剂功能的天然多糖。方法自中药提取当归多糖、黄芪多糖、板蓝根多糖、茯苓多糖、黄精多糖、枸杞多糖和淫羊藿多糖。苯酚硫酸法测总糖含量;间羟联苯法分析糖醛酸含量;凝胶渗透色谱法测定多糖分子量分布。按照分组,每只BALB/c小鼠肌内注射60μg卵清蛋白(OVA),OVA+0.1mg氢氧化铝,OVA+1mg多糖,OVA+生理盐水,并分别在第1,28和58天进行3次注射,在第21,52,70天采血,测定血清OVA抗体滴度。结果 7种多糖中的总糖含量为板蓝根总糖,65.41%;当归总糖,30.88%;黄芪总糖,43.70%;黄精总糖,48.88%;茯苓总糖,58.68%;枸杞总糖,45.83%;淫羊藿总糖,32.60%;总糖中糖醛酸含量为板蓝根糖醛酸,13.36%;当归糖醛酸,19.73%;黄芪糖醛酸,6.53%;黄精糖醛酸,5.96%;茯苓糖醛酸,1.96%;枸杞糖醛酸,8.96%;淫羊藿糖醛酸,7.53%。7种多糖表现出不同的分子质量分布特征。初次免疫,7种多糖和铝佐剂均未激活小鼠血清OVA抗体产生;在第2次免疫后,淫羊藿多糖佐剂组产生较高滴度的抗体,达到1:105;第3次免疫后淫羊藿多糖佐剂组抗体滴度进一步提高,板蓝根多糖、当归多糖及茯苓多糖佐剂组的OVA特异性抗体滴度均达到1:105,与OVA+0.1mg氢氧化铝及OVA+生理盐水组比较有显著差异(P<0.05)。结论板蓝根多糖、当归多糖、茯苓多糖及淫羊藿多糖均具有很好的佐剂作用,特别是淫羊藿多糖具有较强的激发体液免疫活性。多糖有望成为候选的新型免疫佐剂。OBJECTIVE To find good adjuvant candidates from natural polysaccharides.METHODS Astragalus polysaccharide（APS）,Angelica root polysaccharides（ARPS）,Epimedium polysaccharides（EPS）,Isatis root polysaccharide（IRPS）,Lycium polysaccharides（LCPS）,Poria polysaccharides（PPS） and Solomonseal polysaccharides（SPS） were extracted,respectively.The carbohydrate contents were determined by phenol-sulfuric acid method.The uronic acids contents of these polysaccharides were measured by m-hydroxylbiphenyl assay.The molecular mass characters were identified by gel permeation chromatography.BALB /c mice were intramuscularly injected with ovalbumin（OVA） 60 μg,OVA 60 μg mixed with aluminum hydroxide（0.1 mg） or polysaccharide（1 mg） respectively on day 1,28 and 56.Serum samples were collected on day 21,52 and 70 for measurement of anti-OVA special antibody.RESULTS The carbohydrate contents（%） were 65.41 for IRPS;30.88 for ARPS;43.70 for APS;48.88 for SPS;58.68 for PPS;45.83 for LCPS;32.60 for EPS.The uronic acids contents（%） were 13.36 for IRPS;19.73 for ARPS;6.53 for APS;5.96 for SPS;1.96 for PPS;8.96 for LCPS;7.53 for EPS.The results of molecular mass showed different distritutiion characters among these polysaccharides.The initial immunity with seven polysaccharides and alum did not elicit significant immune responses against OVA.After the second immunization,only EPS induced a high level of OVA-specific antibody with the titer of 1∶ 105.After the third time of immunization,the antibody titers induced by EPS were further increased,and the antiOVA antibody titers of mice treated with IRPS,ARPS and PPS reached 1 ∶ 105.The specific antibody levels induced by four polysaccharides were significantly different from those of alum groups and no adjuvant control.CONCLUSION Four polysaccharides,IRPS,ARPS,PPS and EPS,exhibited good adjuvant activities.EPS showed strong potential to increase humoral immune responses.They could be efficacious candidates for new adjuvants.

关 键 词：多糖 当归 多糖 黄芪 多糖 板蓝根 多糖 茯苓 多糖 黄精 多糖 枸杞 多糖 淫羊藿 免疫 佐剂 

分 类 号：R285.5[医药卫生—中药学]