双酚A对大鼠卵泡体外生长和卵母细胞成熟的影响  

Effect of bisphenol A on rat follicle growth and oocyte maturation in vitro

作　　者：夏仪[1] 王卓[1] 张天宝[1] 

机构地区：[1]第二军医大学毒理学教研室,上海200433

出　　处：《中国药理学与毒理学杂志》2013年第3期423-428,共6页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金(30571587)~~

摘　　要：目的观察双酚A(BPA)对大鼠卵泡体外生长发育及卵母细胞成熟的影响。方法采用大鼠卵泡体外长期培养方法,从12～14d龄的雌性大鼠卵巢中机械性分离腔前卵泡(140～170μm),隔天分别换一半含BPA 0,50,100和150μmol·L-1的培养液,连续培养10d。倒置相差显微镜下观察卵泡发育的形态,计算卵泡存活率、有腔卵泡形成率和卵丘-卵母细胞复合体(COC)排出率,测定卵泡直径,显微镜下观察卵泡的排卵情况以及卵母细胞成熟情况,计算生发泡(GV)、生发泡破裂(GVBD)和第一极体(PB)的形成率;分别于培养2,6和10d时采用磁性酶联免疫法测定培养基中雌二醇和孕酮的分泌量。结果正常对照组卵泡在10d培养过程中,大多数正常对照组卵泡都经历了腔前卵泡、有腔卵泡以及成熟卵泡阶段。与正常对照组相比,BPA 100和150μmol·L-1组的卵泡存活率、有腔卵泡形成率、COC排出率、GVBD率以及PB率均明显降低(P<0.05)。BPA 50μmol·L-1组培养10d时的卵泡直径以及培养6和10d时BPA100和150μmol·L-1组卵泡和卵母细胞的直径均明显降低(P<0.05);与正常对照组相比,BPA 100和150μmol·L-1组卵泡膜细胞和颗粒细胞的增殖受到明显抑制(P<0.05)。与正常对照组相比,BPA 100和150μmol·L-1组在培养6和10d时雌二醇和孕酮含量均显著减少(P<0.01)。结论 BPA 100和150μmol·L-1可抑制大鼠卵泡的生长和卵母细胞的成熟。OBJECTIVE To observe the effect of bisphenol A（BPA） on rat follicle in vitro growth and oocyte maturation.METHODS Using in vitro long-term culture method rat follicles,preantral follicles（140-170 μm） were mechanically isolated from 12-14-day-old female rats and randomly divided into BPA 50,100,150 μmol·L-1 groups and normal control group,and then cultured for 10 d.Then the morphology of follicle development was observed under a microscope and follicle survive antrum cumulus-oocyte cell complexes（COC）,germinal vesicle breakdown（GVBD） and first polar body（PB） rates were calculated,steroid hormone production,ovulation rate and oocyte maturation rate to evaluate the effect.RESULTS During 10 d of culture,follicles in normal control group the preantral follicles,antral follicles and mature follicle stage.Compared with normal control group,follicle diameter at 10 d in BPA 50 μmol·L-1 group decreased（P〈0.05）,follicle diameter and docyte diameter in BPA 100 and 150 μmol·L-1 groups at 6 and 10 d were reduced（P〈0.05）.Compared with normal control group,the rates of follicle survival,cavity formation,the release of COC and PB in BPA 100 and 150 μmol·L-1 groups statistically decreased（P〈0.05）.In BPA 100 and 150 μmol·L-1 groups,the proliferation of theca cells and granulosa cell were suffered a different degree of inhibition（P〈0.05）.Compared with normal control group,the secretion of estradiol and progesterone in BPA 100 and 150 μmol·L-1 groups were statistically reduced（P〈0.01）.CONCLUSION BPA≥100 μmol·L-1 can inhibit follicle development and oocytes mature.

关 键 词：双酚A 生殖毒性 卵泡 体外培养 

分 类 号：R96[医药卫生—药理学]