大鼠股骨缺损模型中BBP增强BMP-2的骨诱导作用  

Enhancement of BMP binding peptide combined with recombinant human BMP-2 on the heal of a rodent segmental femoral defect

作　　者：费志强[1,2,3,4,5] 熊建义[1,2] 刘志勇[1,2] 黄江鸿[1,2] 王大平[1,2] 沈慧勇[3] 黄德民[4,5] 梁加利[4,5] 

机构地区：[1]深圳市第二人民医院骨科,518035 [2]深圳市第二人民医院深圳市组织工程重点实验室,518035 [3]中山大学孙逸仙纪念医院骨科,广州510120 [4]香港大学深圳医院骨科,518053 [5]香港大学深圳医院深圳市骨科创伤修复新技术重点实验室,518053

出　　处：《中国矫形外科杂志》2013年第12期1215-1222,共8页Orthopedic Journal of China

基　　金：中国博士后基金面上资助项目(编号:2011M501365);广东省自然科学基金面上项目(编号:S2012010008129);深圳市重点实验室提升项目(编号:ZDSY20120614154551201);国家留学基金委员会公派留学资助;广东省医学科研基金(编号:B2013351)

摘　　要：[目的]验证在大鼠节段性骨缺损模型中BBP对于rhBMP-2骨诱导作用的影响。[方法]70只缺损分别分成7组,每组不同剂量的rhBMP-2+/_1 000μg BBP,4、8周后分别摄片,动物8周后处死,股骨样本分别手工评估,采用uCT测量骨容积,随后分别采用组织学和生物力学分析。[结果]高剂量(10μg)rhBMP-2组术后8周可见骨愈合,骨缺损处骨的完全覆盖和桥接,但低剂量(5μg和2μg)rhBMP-2组术后8周骨愈合欠佳。与单独应用rhBMP-2相比,使用低剂量的rhBMP-2复合一定量的BBP可以取得更满意的骨形成量。BBP增强rhBMP-2的骨形成活性发生于4～8周时,而在术后早期并无明显作用。单纯应用BBP仅可见骨缺损处局部钙化,未见骨愈合。[结论]BBP能显著增强rhBMP-2的骨形成活性,这种增强作用需要一定时间来产生效果;其活性发生于术后4～8周时,在术后早期并无明显作用。而且BBP本身并没有骨诱导潜力,仅仅能增强rhBMP-2的骨形成活性。BBP起到缓释作用,与rhBMP-2紧密结合后,让rhBMP-2缓慢而持久的释放。[ Objective] To elucidate the potential enhanced efficacy of BBP using critical-sized segmental femoral bone defects in rats. [ Method] Seventy defects in seven groups of rats were filled with various amounts （0, 2, 5, and 10 μg） of rhBMP-2 with or without 1 000 μg BBP. Radiographs were obtained after 4 and 8 weeks. The animals were euthanized at 8 weeks, and femoral specimens were assessed manually, evaluated for bone volume using microcomputed tomography, and subjected to histological or biomechanical analysis. [ Result] Although 10 μg rhBMP-2 yielded consistent results in terms of bone healing and quality of bone repair across the segmental defect, lower doses of rhBMP-2 failed to induce satisfactory bone healing. However, the combined administration of lower doses of rhBMP-2 and BBP induced the formation of significantly large amounts of bone. [ Conclusion] Our results suggest that the combined administration of rhBMP-2 and BBP facilitate bone healing and have potential clinical applications.

关 键 词：BMP-2 BBP 股骨缺损 骨愈合 

分 类 号：R687.3[医药卫生—骨科学]