巨脾大部切除术后残脾T、B淋巴细胞分布与定量研究  

T and B lymphocytes in residual spleen after subtotal splenectomy for splenomegaly

作　　者：褚海波 张廷国[2] 赵建华 徐永波 王涛 王敏 菅凤国 唐金元 孙宏杰 李坤 郭文君[3] 

机构地区：[1]解放军第八九医院普外中心,潍坊261021 [2]潍坊医学院研究生部 [3]潍坊医学院病理教研室 [4]昌邑人民医院普外科

出　　处：《中华肝胆外科杂志》2013年第6期429-432,共4页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的探讨巨脾大部切除后残脾组织内T、B淋巴细胞浸润情况。方法收集门静脉高压症患者巨脾标本13例，术后8年穿刺获取残脾标本13例，另设外伤性脾组织13例作对照。采用免疫组化染色，光镜下观察T、B淋巴细胞分布并计数。结果T、B淋巴细胞在残脾组分别为（59．769士9．429）个／10。像素面积、（10．822士1．938）个／10^-5像素面积；巨脾组为（34．715±3．497）个／10^-5像素面积、（2．369±0．664）个／10^-5像素面积；对照组为（48．229±14．869）个／10^-5像素面积、（6．844±0．807）个／10^-5像素面积。残脾组与巨脾组与对照组比较差异有统计学意义（P〈0．05），巨脾组与对照组比较差异亦有统计学意义（P〈0．05）。结论巨脾大部切除后，残脾单位面积T、B淋巴细胞数目明显增加，提示残脾的免疫功能可能有所改善。Objective To investigate the infiltrative changes of T and B lymphocytes in residual splenic tissues after subtotal splenectomy for splenomegaly. Methods Thirteen patients with splenomegaly due to portal hypertension were studied. The specimens were obtained by operative and percutaneous puncture biopsy. There were 2 groups of specimens from this group of patients： the splenomegaly and residual spleen groups. The control group consisted of 13 patients with traumatic rupture splenic tissues. The samples were sliced, and the distribution and counts of T and B lymphocytes were observed under light microscopy using immunohematological staining. Results In the residual spleen group, the numbers of T and B cell were （59. 769±9. 429） per 10-5 pixel area, （10. 822±1. 938） per 10^-5pixel area, while in the splenomegaly group they were （34. 715±3. 497） per 10^-5 pixel area, （2. 369±0. 664） per 10^-5 pixel area, and in the control group they were （48. 229±14. 869） per 10 5 pixel area, （6. 844±0. 807） per 10^-5 pixel area, respectively. A significantly higher number of T and B cells were observed in the residual spleen group when compared with the splenomegaly group and the control group （P〈0.05）. Significantly lower numbers of T and B cells were observed in the splenomegaly group when compared with the control group （P〈0.05）. Conclusions T and B lymphocytes increased significantly per unit area in the residual spleen after subtotal splenectomy. The results suggested that the immune function of the residual spleen can be improved.

关 键 词：脾大 脾切除术 脾功能亢进 淋巴细胞 

分 类 号：R657.6[医药卫生—外科学]