两种方法测定血氨分析性能的比较与评价  

Performance and Comparation ofTwo Methods forDetection of BloodAmmonia

作　　者：任占宝[1] 王金英[1] 

机构地区：[1]首都医科大学附属北京朝阳医院京西院区检验科,北京100043

出　　处：《继续医学教育》2013年第5期55-58,共4页Continuing Medical Education

摘　　要：目的:探讨血氨不同检测方法结果的差异,以便根据临床需要选择合适的方法测定血氨。方法:用英国郎道公司生产的血氨试剂和日本京都血氨测定仪分别用酶速率法和干化学法测定50例血氨浓度,分析两种方法的性能及检测结果相关性。结果:干化学法与临床诊断有很好的总符合率,其总符合率达94%,且灵敏度、特异性较高,误诊率和漏诊率低。两种检测方法测定结果之间有统计学意义,P=0.004。酶速率法与干化学法相关性Y=0.904X+1.783 2,回归方程决定系数R2=0.959 1,相关系数r=0.971。结论:通过本次评价可知干化学法在分析性能上优于酶速率法,适用于急诊及床旁检测。Object： To investigate the difference and correlation of the results of two method based on different principal measuring blood ammonia, so as to provide evidence to choose suitable method to meet clinic demands. Methods： 50 samples were measured by both methods of enzymic rate method and dry-chemical analysis. The reagents of enzymic rate method are from the Randox Company, Britishi, while the reagents and instrument of dry-chemical analysis from the Arkray Company, Japan. Performances of two methods were studied and correlate was made statistically Results： The results of dry-chemical analysis matches the clinical diagnosis verification very we with high coincidence rate of 94%. The sensitivity and specificity of the dry-chemical analysis is higher, while the mis-diagnosis rate and wrong diagnesis rate is quite lower than the enzymic rate method. The difference between the two methods is distinguished. The results of dry-chemica analysis（Y） and enzymic rate method（X） correlated well with each other Y = 0.904X＋1.783 2 （r=0.971 R2=0.959 1）. Conclusions： Performances of the dry-chemical analysis of measuring blood ammonia is superior to that of enzymic rate method in terms of emergent test and POCT （Point of Care Testing）.

关 键 词：血氨 酶速率法 干化学法 性能评价 

分 类 号：R446[医药卫生—诊断学]