山茱萸总苷及其含药血清对MSCs增殖的影响  

Effect of total Glucosides from Cornus officinalis and its medicated serum on the MSCs proliferation

作　　者：王艳[1] 宋春静[1] 孙秀岩[1] 曹颖[1] 李晶[2] 毕月玲[1] 

机构地区：[1]天津中医药大学,天津市中药化学与分析重点实验室,300193 [2]天津中医药大学第一附属医院,300193

出　　处：《天津中医药大学学报》2013年第2期101-104,共4页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：国家自然基金项目(30901988);天津市高等学校科技发展基金计划项目(20110206)

摘　　要：[目的]探讨山茱萸总苷及其含药血清对骨髓间充质干细胞(MSCs)增殖的影响。[方法]分离、培养大鼠MSCs,分别加入山茱萸总苷及其含药血清,运用四甲基偶氮唑蓝(MTT)法检测不同浓度山茱萸总苷及其含药血清对大鼠MSCs增殖的影响。[结果]大鼠灌胃1 h后采血所得含药血清以20%的浓度干预时具有最佳的促MSCs增殖作用,山茱萸总苷最佳促MSCs增殖浓度为625μg/mL。[结论]山茱萸总苷及其含药血清可促进大鼠MSCs增殖。[Objective] To discuss the effect of total Glucosides from Comus officinalis and its medicated serum on proliferation of mesenchymal stem cells （MSCs）.[Methods] MSCs were isolated from rat bone and cultured under total G[ueosides from Comus officinalis and its medicated serum, the proliferation effect on MSCs was detected with MTT.[Results] The 20% concentration of the TGC serum collected from rats 1 h later after intragastric infusion was proved to be the most effective concentration on the proliferation of MSCs, the 625 μg/mL concentration of the TGC was proved to be the most effective concentration on the proliferation of MSCs. [Conclusion] The total Glucosides from Comus officinalis and its medicated serum can promote the proliferation of MSCs in rats.

关 键 词：山茱萸总苷 含药血清 骨髓间充质干细胞 细胞增殖 

分 类 号：R285.5[医药卫生—中药学]