溶剂萃取分离S-(+)-DMCPA酶法制备液中底物和产物的研究  

Study on S-(+)-2,2-dimethylcyclopropanecarboxylic acid separation and substrate recovery from enzymatic conversion solution

作　　者：黄金[1] 梁法勇[1] 何军邀[1,2] 钟成刚[1] 王普[1] 

机构地区：[1]浙江工业大学药学院,浙江杭州310032 [2]浙江医药高等专科学校,浙江宁波315100

出　　处：《浙江工业大学学报》2013年第3期270-274,共5页Journal of Zhejiang University of Technology

基　　金：浙江省科技厅重大科技攻关项目(2010C11040);浙江省教育厅科研项目(Y201018521)

摘　　要：采用分步溶剂萃取法对脂肪酶Novozyme 435催化2,2-二甲基环丙烷甲酸乙酯(DMCPE)不对称水解得到的S-(+)-2,2-二甲基环丙烷甲酸(S-(+)-DMCPA)制备液进行萃取分离.结果表明,采用等体积正己烷为萃取剂,在35℃和200r/min下,对酶法制备液萃取3次,每次萃取40min,底物DMCPE的萃取收率可达99.2%;继而采用等体积的乙酸乙酯在30℃,200r/min条件下,对萃余液进行2次萃取,每次萃取30min,产物S-(+)-DMCPA的萃取收率为99.5%.该研究对从酶法制备液中实现产物S-(+)-DMCPA的分离和未转化底物DMCPE的回收再利用,以及今后该分离过程的放大具有一定的借鉴和指导意义.The methods for S-（＋）-DMCPA separation and substrate （DMCPE） recovery from enzymatic conversion solution were investigated. Firstly, DMCPE was extracted thrice with an equal volume of N-hexane at 35 ℃ and 200 r/min for 40 min. With these promoted methods, 99.2% recovery yield of unreacted substrate was obtained. Subsequently, in order to harvest an higher product yield, the extraction raffinate was further extracted twice with an equal volume of ethyl acetate at 30 ℃ and 200 r/min for 30 rain. The yield of S-（＋）-DMCPA reached 99.5%. In this investigation, an efficient means for separation of product S-（＋）-DMCPA and recycling of unreacted substrate DMCPE from enzymatic conversion solution was developed. The results can also provide reference for the scale-up process of separation.

关 键 词：分离 S-(+)-2  2-二甲基环丙烷甲酸 溶剂萃取 2  2-二甲基环丙烷甲酸乙酯 

分 类 号：TQ460.64[化学工程—制药化工]