苎麻与大麻CesA1基因的生物信息学分析  

Bioinformatics Analysis of Cellulose Synthase Gene (CesA1) of Boehmeria nivea L. and Cannabis Sativa L.

作　　者：陈平[1] 喻春明[1] 王延周[1] 陈继康[1] 熊和平[1] 

机构地区：[1]中国农业科学院麻类研究所,湖南长沙410205

出　　处：《中国麻业科学》2013年第3期118-121,154,共5页Plant Fiber Sciences in China

基　　金：国家科技支撑计划(2010BAD02B01);中国农业科学院基本科研业务项目(1610032012030)

摘　　要：本文利用生物信息学方法从转录组测序数据库中分离了苎麻纤维素合成酶CesA1(BnCesA1)基因全长编码区序列(NCBI登录号:KC112993),并与大麻纤维素合成酶(CsCesA1)进行了氨基酸组成成分、理化性质以及二级结构比较,构建了CesA1基因家族的进化树。结果显示BnCesA1由3249个碱基组成,编码1082个氨基酸组成的蛋白,CsCesA1由3198个碱基组成,编码1065个氨基酸组成的蛋白。它们是疏水性脂溶蛋白,都含有6个跨膜区,二级结构以α-螺旋和无规卷曲为主,存在小部分β-转角或扩展链。两基因的核酸序列同源性为86%,氨基酸序列同源性为93%。本文结果为麻类作物纤维素合成酶基因的进一步功能分析提供了基础。The full length of BnCesA1 cDNA coding region was isolated from transcriptome sequen-cing data by bioinformatics method, and logined al parameters of BnCesA1 and CsCesA1 including pological structure of transmembrane regions, were predicted. The results showed that BnCesA1 in GeneBank （accession number： KCl12993） . Sever-sequences composition, physicochemical property, to-hydrophobicity or hydrophilicity, secondary structures ORF was 3249 bp in length, encoded a protein of 1082 amino acids; and CsCesA1 was 3198 bp, encoded a protein of 1065 amino acids. The two genes showed the similarity of 86% in nucleotides and 93% in protein, and the secondary structure was quite similar. The results of this investigation provided a significant foundation for further research on CesA genes of fi-ber crops.

关 键 词：纤维素合成酶 苎麻 大麻 生物信息学分析 

分 类 号：S563.1[农业科学—作物学]