猪带绦虫六钩蚴TSO45-4B抗原FnⅢ结构域相应的线性B细胞表位肽免疫原性研究  

Research on immunity response about Taenia solium oncosphere TSO45-4B antigens FnⅢ structure domain linear B cells epitope peptides

作　　者：王媛媛[1] 陶志勇[1] 杨小迪[1] 王小莉[1] 常雪莲[1] 陈勇[1] 孙新[1] 夏惠[1] 方强[1] 

机构地区：[1]蚌埠医学院病原生物学教研室安徽省感染与免疫重点实验室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2013年第5期513-515,共3页Journal of Bengbu Medical College

基　　金：国家自然科学基金资助项目(30600518);安徽省教育厅自然科学研究资助项目(KJ2011B095);蚌埠医学院校级课题(BY0804;BY1001)

摘　　要：目的:观察载体蛋白偶联的TSO45-4B抗原FnⅢ结构域相应的线性B细胞表位肽诱导的体液免疫反应。方法:人工合成TSO45-4B抗原FnⅢ结构域2条预测表位肽,偶联钥孔血蓝蛋白免疫小鼠,采用ELISA法检测小鼠血清中预测表位肽特异性抗体滴度。结果:免疫小鼠血清中检测到1条预测表位肽特异性抗体,其效价达到1∶1 280。结论:设计的1条TSO45-4B抗原FnⅢ结构域线性B细胞表位肽可诱导小鼠产生体液免疫反应。Objective： To observe the humoral immune response induced by Taenia solium oncosphere TSO45-4B antigens FnⅢ structure domain linear B cells epitope peptides in mice.Methods： The two predicted B cell epitope peptides of TSO45-4B Fn Ⅲ structure domain conjugated with carrier protein of keyhole limpet hemocyanin were synthesized and used to immunize mice.The mice specific serum antibody titer to the epitope peptides synthesized was tested by ELISA.Results： The specific antibody to one of the predicted epitope peptides synthesized was found in mice serum,and the titer was 1∶ 1 280.Conclusions： One of the two predicted linear B cell epitope peptides of TSO45-4B FnⅢ structure domain can induce the humoral immune response in mice.

关 键 词：猪带绦虫 六钩蚴 TSO45-4B FnⅢ结构域 B细胞表位 免疫反应 

分 类 号：R383.3[医药卫生—医学寄生虫学]