日本血吸虫重组抗原rSjGALE对小鼠的免疫保护效果分析  

IMMUNOPROTECTIVE EFFECT INDUCED BY RECOMBINANT ANTIGEN RSJGALE OF SCHISTOSOMA JAPONICUM

作　　者：曹宇凡[1] 乔洪宾[1] 刘萍萍[1] 杨云霞[1] 石耀军[1] 李浩[1] 刘金明[1] 林矫矫[1] 金亚美[1] 

机构地区：[1]中国农业科学院上海兽医研究所农业部动物寄生虫重点实验室,上海200241

出　　处：《中国动物传染病学报》2013年第3期51-56,共6页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然科学基金项目(30800819);中央级公益性科研院所基本科研业务费项目(2012ZL091;2013JB17)

摘　　要：前期研究结果表明日本血吸虫(Schistosoma japonnicum)UDP-葡萄糖表异构酶(GALE),在抗血吸虫病方面有一定的免疫保护效果,尤其是对雌虫产卵有较大影响。为制定更适宜的免疫保护方案,本研究选用206佐剂与rSjGALE重组蛋白联合免疫动物,结果表明,该蛋白与206佐剂联用有一定的免疫保护作用,免疫后小鼠产生了特异性抗体,且在减虫、减卵、减孵化等方面均有一定作用。In a previous study,the immunoprotective effect of UDP-Glucose-4-Epimerase protein of Schistosoma japonicum（SjGALE） was demonstrated,especially in reducing female spawning.In the present study,a combined use of rSjGALE and montanide ISA 206 was tried to see more potent immunoprotection.The results showed that the combination of rSjGALE and montanide ISA 206 induced specific antibodies and reduced liver egg burden,female spawning and hatching rate.However,their immunoprotective activity was not as stable as Freund adjuvant.

关 键 词：日本血吸虫 SjGALE 206佐剂 免疫保护 

分 类 号：S852.735[农业科学—基础兽医学]