在固体基质表面制备具有催化活性的含酶分子多层膜  

Fabrication of Multilayer Film with Catalytic Activity on the Solid Substrate Surface

作　　者：李文江[1] 周兵[1] 沙晓祥[1] 孙长青[1] 王子忱[1] 赵慕愚[1] 向前[2] 

机构地区：[1]吉林大学化学系,长春130023 [2]长春工程学院化学系,长春130021

出　　处：《吉林大学自然科学学报》2000年第4期75-78,共4页Acta Scientiarum Naturalium Universitatis Jilinensis

摘　　要：在固体基质表面自组装一层功能分子层 ,通过静电相互作用 ,将辣根过氧化物酶( HRP)与双吡啶盐 ( Py C6 BPC6 Py)交替沉积 ,制得多层酶电极 .用紫外可见光谱法跟踪石英基片上的组装过程 .详细描述多层膜电极以亚甲绿 ( MGH)为电子中继体的电化学行为 。A new amperometric enzyme sensor has been produced based on ionic self assembly technique. The assembly process was monitored by ultraviolet visible spectroscopy on quartz slides. The diffusing mediator methylene green (MGH) as electron shuttle in a solution was able to transfer electrons successfully between the active center of the immobilized HRP and the electrode surface. The enzyme electrode was sensitive to the reduction of hydrogen peroxide.

关 键 词：辣根过氧化物酶 分子多层膜 电催化 传感器 

分 类 号：O657.1[理学—分析化学] Q－39[理学—化学]