低温胁迫后不同光强对小黑杨幼苗叶片叶绿素荧光和能量分配的影响  

Effects of Lights Intensity on Chlorophyll Fluorescence Characteristics and Energy Allocation Pathways in Leaves of Populus simonii×P.nigra seedlings after Chilling Stress

作　　者：宋扬[1] 张潆心[1] 郭娜[1] 孙广玉[1] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《安徽农业科学》2013年第10期4421-4423,共3页Journal of Anhui Agricultural Sciences

基　　金：东北林业大学本科创新项目(201210225107)

摘　　要：[目的]研究低温胁迫后不同光强对小黑杨幼苗叶片叶绿素荧光和能量分配的影响,提高幼苗移栽成活率。[方法]以小黑杨幼苗为试验材料,利用叶绿素荧光技术研究了低温(4～6℃)胁迫3 h后不同光强(200、1 200μmol/(m2.s)对小黑杨幼苗叶片光合特性的影响。[结果]200μmol/(m2.s)的弱光下,低温胁迫后小黑杨幼苗叶片的各叶绿素荧光参数与常温处理之间差异较小,但1 200μmol/(m2.s)的强光下,低温胁迫后小黑杨幼苗叶片的实际光化学效率(ФPSⅡ)和电子传递速率性(ETR)明显低于常温处理,并且叶片的最大荧光(Fm)、PSⅡ最大光化学效率(Fv/Fm)和潜在光化学活性(Fv/Fo)也明显降低。另外,低温胁迫后弱光处理对小黑杨幼苗叶片的光能分配参数影响不大,但强光引起了低温胁迫后小黑杨幼苗叶片光能分配的紊乱,PSⅡ反应中心吸收光能用于光化学反应的比例明显降低,失活反应中心耗散的光能比例明显增加,说明强光是引起低温胁迫后小黑杨幼苗叶片发生光抑制的重要原因之一。[结论]春季小黑杨幼苗移栽时,低温逆境发生后要注意采取措施进行遮阴处理,降低叶片的光抑制程度,以提高幼苗移栽的成活率。In this paper,taking Populus simonii×P.nigra as the test material,effects of different lights(200,1 200 μmol/(m2·s) on chlorophyll fluorescence characteristics of Populus simonii×P.nigra after low temperature(4-6 ℃) were studied.[Result] Little differences between Populus simonii×P.nigra after low temperature and normal temperature were found in chlorophyll fluorescence parameters under low light 200 μmol/(m2·s).The actual photochemical efficiency(ФPSⅡ) and the electron transport rate(ETR) in leaves of Populus simonii×P.nigra seedlings after low temperature were lower after normal temperature under high lihgt,and maximum fluorescence(Fm),PSⅡphotochemical efficiency(Fv/Fm) and potential activities(Fv/Fo) also decreased significantly.In addition,leaves of Populus simonii×P.nigra after low temperature under low light has little effect on the PSⅡenergy allocation pathways.But high light led the disorder of the PSⅡenergy allocation pathways in leaves of Populus simonii×P.nigra after low temperature.The quantum yield of photochemistry in the PSⅡenergy decreased,quantum yield of thermal dissipation associated with the presence of non-functional PSⅡsignificantly increased.This explained high light was one of the important reasons why indured photoinhibition after low temperature in leaves of Populus simonii×P.nigra.[Conclusion] When Populus simonii×P.nigra seedlings transplant in spring,shading is a measure after low temperature stress which can alleviate the photoinhibition of leaves for increase transplanting survival rate of Populus simonii×P.nigra seedlings.

关 键 词：小黑杨 低温 叶绿素荧光 光强 

分 类 号：S792.11[农业科学—林木遗传育种]