后肢去负荷干预后不同时间的跑台训练对大鼠股骨骨密度和骨代谢指标的影响  

Effect of Treadmill Training at Different Time after Hindlimb Unloading on Bone Mineral Density of Rat Femurs and Markers of Bone Metabolism

作　　者：王晓倩[1] 张卫平[2] 

机构地区：[1]太原工业学院体育部,山西太原030008 [2]浙江工业大学体育军训部,浙江杭州310014

出　　处：《北京体育大学学报》2013年第5期64-68,共5页Journal of Beijing Sport University

基　　金：山西省高等学校哲学社会科学研究一般项目(20112227);山西省社科联重点课题研究项目(SSKLZDKT2011053)

摘　　要：目的:研究后肢去负荷干预后不同时间的跑台训练对大鼠股骨骨密度和骨代谢指标的影响。方法:9周龄Wistar雌性大鼠64只随机分为8组,每组8只。Group 1:尾部悬吊4周,处死;Group 2和Group3:尾部悬吊4周,之后分别跑台训练4周和8周,处死;Group 4和Group 5:尾部悬吊4周,之后分别自由活动4周和8周,处死;Group 6、Group 7和Group 8:正常饲养,不进行尾部悬吊,分别于分组4周后、8周后和12周后处死。测试各组大鼠股骨骨密度和血清骨代谢指标。结果:与其对应周龄的阴性对照组相比,尾部悬吊组大鼠血钙和抗酒石酸酸性磷酸酶5b显著升高,血清碱性磷酸酶和骨钙素无显著差异,骨密度显著降低;恢复正常活动后,大鼠血清碱性磷酸酶和骨钙素显著升高,血钙和抗酒石酸酸性磷酸酶5b显著下降,骨密度显著升高;与自由活动组相比,跑台训练组大鼠上述骨代谢指标和骨密度的变化更显著。结论:后肢去负荷导致大鼠骨吸收作用显著增强,使骨密度下降;后肢恢复正常活动后,大鼠的骨形成作用显著增强,并且骨吸收作用也逐渐降至正常水平,骨密度逐渐升高;跑台训练可加速大鼠后肢去负荷干预后骨代谢的改变和骨密度的恢复。Objective： The purpose of this study is to study the effect of treadmill training at different time after hindlimb unloading on bone mineral density of rat femurs and markers of bone metabolism. Method： Sixty-four Wistar rats aged 9 weeks were used and randomly divided into eight experimental groups ： Group 1, the suspended control under hindlimb unloading regimen for 4 weeks, then euthanized ; Groups 2 and 3, the suspended subjected to hindlimb unloading for 4 weeks, followed by treadmill exercise for 4 weeks （ Group 2） or 8 weeks （ Group 3 ） , then euthanized ; Groups 4 and 5, designated free suspended under hindlimb unloading regimen for 4 weeks fol- lowed by free activity in cages for 4 weeks （ Group 4） or 8 weeks （ Group 5 ） , then euthanized ; Groups 6, 7 and 8, negative controls allowed to free activity in cages and euthanized 4, 8 and 12 weeks after grouping respectively. Bone mineral density （BMD） of the left femur and markers of bone metabolism was analyzed. Results ： Compared with the rats of corresponding negative control group, serum calcium and tartrate-resistant acid phosphatase 5b of the rat of tail suspension group was significantly elevated, serum alkaline phosphatase and osteoealcin was no sig- nificant difference, bone mineral density was significantly reduced; After returning to normal activity, serum alka- line phosphatase and osteoealcin hormone significantly increased, calcium and tartrate-resistant acid phosphatase 5b significantly decreased, bone mineral density significantly increased; Compared with the free group, the above indicators of bone metabolism and bone mineral density of the treadmill training group changes more significant. Conclusion： hindlimb unloading lead to significantly enhanced bone resorption in rats and decreased bone mineraldensity ; After returning to normal activities, the rat bone formation be enhanced significantly and hone resorption is gradually reduced to normal levels, bone density increased gradually; Treadmill training can ac

关 键 词：后肢去负荷 康复时间 跑台训练 骨密度 骨代谢 

分 类 号：R580[医药卫生—内分泌]