Effects of Antibiotics on Seed Germination of Different Rape Cultivars  

卡那霉素和潮霉素对不同类型油菜种子萌发的影响研究(英文)

作　　者：刘建民[1,2] 尹永泰 李红[2] 栗茂腾[2] 

机构地区：[1]河南理工大学资源环境学院,河南焦作454000 [2]华中科技大学生命科学与技术学院,湖北武汉430074

出　　处：《Agricultural Science & Technology》2013年第5期707-709,721,共4页农业科学与技术（英文版）

基　　金：Supported by the National High Technology Research and Development Program of China(863Program,2007AA10Z120);the Doctoral Fund of Henan Polytechnic University(B2011-035)~~

摘　　要：[Objective] The research aimed to study the seed germination and growth of different rape cultivars, including Brassica napus, Brassica juncea and Brassica rapa, treated with kanamycin or hygromycin at different concentrations. [Method] Brassica napus Chun K101, Brassica napus L0935 and Brassica rapa L0926 seeds were cultured on 1/2 MS medium supplemented with different concentrations of antibiotics. The hypocotyl and taproot changes of different rape cultivated were recorded and analyzed. [Result] It was shown that 50 mg/L kanamycin could stimulate rapeseed hypocotyl growth, but obviously restrain taproot development. Hygromycin apparently inhibited the growth of hypocotyl and taproot of all the three cultivars. [Conclusion] Kanamycin was suitable to screen the transformed seed of Brassica juncea. The optimal concentration of hygromycin to screen transgenic rapeseed was 40-60 mg/L.[目的]研究卡那霉素（Kan）和潮霉素（Hyg）对甘蓝型油菜、芥菜型油菜和白菜型油菜种子萌发和幼苗生长的影响。[方法]将甘蓝型油菜春K101、芥菜型油菜L0935和白菜型油菜L0926种子分别播种在含有不同浓度Kan或Hyg的1/2MS培养基上,比较不同条件下油菜下胚轴和主根生长的变化。[结果]50mg/L的Kan对油菜的下胚轴生长有促进作用,但对主根的生长有较明显的抑制。Kan对芥菜型油菜种子萌发和幼苗生长的影响最大。Hyg对不同类型油菜的下胚轴和主根生长都有明显的抑制作用。[结论]Kan可用于芥菜型油菜遗传转化实验中转化体的筛选,Hyg对转基因油菜种子筛选的最佳浓度为40~60mg/L。

关 键 词：Brassica napus Brassica juncea Brassica rapa Antibiotic Seed germination 

分 类 号：S565.4[农业科学—作物学]