高效大豆根瘤菌筛选的初步研究  

Preliminary Study on Screening of Efficient Soybean Rhizobium

作　　者：吴萍[1] 何庆元[1] 李正鹏[1] 史钧[1] 祝嫦巍[1] 

机构地区：[1]安徽科技学院应用微生物研究所,安徽凤阳233100

出　　处：《安徽科技学院学报》2013年第3期29-32,共4页Journal of Anhui Science and Technology University

基　　金：安徽省教育厅自然科学基金项目(KJ2011Z066);安徽省教育厅自然科学基金项目(KJ2013A077);安徽科技学院自然科学基金项目(ZRC2013367);安徽科技学院生物学重点建设学科项目(AKXK20102-1)

摘　　要：采用CR-YMA培养基对新鲜的大豆根瘤提取物进行根瘤菌的分离、纯化培养,获菌落为圆形乳白色,表面凸起,直径0.4～0.5cm,革兰反应阴性,显微观察显示为短杆状的根瘤菌26株,并对26个菌株进行蛭石盆栽试验,接种效果显示:接种根瘤菌的大豆植株都有结瘤;与对照相比在结瘤数、鲜瘤重、瘤干重及植株地上部分干重分别增加了14.6%～1330.4%,6.3%～461.9%,28.6%～492.9%,10.2%～86.3%,植株全氮量在9.188%和12.563%之间。初步筛选出与大豆品种"南农1138-2"最佳共生匹配的根瘤菌菌株分别是:AF1、AF9、AH10。26 rhizobium strains were isolated from fresh soybean nodule extracts used CR -YMA medium. All the strain colonies are round and milky white, convex surface; the colony diameters are between 0.4-0.5cm; all the strains are gram-negative and short rod shape. The incubation experiments with soybean plants in vermicu-lite medium showed that all the soybean plants can nodulate; and the number of the nodules, fresh nodule weights, dried nodule weights and the dried weights of the plants above the ground increased 14.6% - 1330.4%, 6.3% - 461.9% ,28.6% - 492.9%, 10.2% - 86.3 % separately compared with the control. The total quantity nitrogen of the plant is between 9. 188% - 12.563%. The rhizobium strains symbiotic matches best with the soy-bean plant Nannong1138-2 are AF1 ,AF9 and AH10.

关 键 词：根瘤菌 大豆 共生固氮 筛选 

分 类 号：S182[农业科学—农业基础科学]