甘草酸和苯巴比妥钠对小鼠肝P450 3A酶的影响  

Effect of glycyrrhizin and phenobarbital pretreatment on hepatic P450 3A enzyme in mice

作　　者：程建峰[1] 滕树保[1] 陈东鸿[1] 贺建荣[1] 王茹[1] 刘新友[1] 贺平[2] 

机构地区：[1]第四军医大学唐都医院药学部,陕西西安710038 [2]第二军医大学东方肝胆外科研究所,上海200438

出　　处：《第四军医大学学报》2000年第8期968-970,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金!资助课题 ( 3 9670 883)

摘　　要：目的　研究甘草酸和苯巴比妥钠对小鼠肝 P45 0 3A酶活性、蛋白含量及 m RNA表达的影响 .方法　给小鼠甘草酸 5 0 mg· kg- 1 、苯巴比妥钠 5 0 mg· kg- 1 或等量注射用水 ig7d后 ,采用利多卡因代谢试验 (MEGX test)、酶联免疫吸咐测定法 (EL ISA)和逆转录聚合酶链反应 (RT- PCR)测定肝微粒体中 P45 0 3A酶活性、蛋白含量及其 m RNA的表达 .结果　与正常对照组相比 ,甘草酸组小鼠尿液 MEGX累积排泄量和肝 P45 0 3A蛋白含量无明显改变 .苯巴比妥钠组小鼠MEGX累积排泄量增加 2 3.4% (P<0 .0 5 ) ,肝 P45 0 3A酶蛋白含量增加 43.4% (P<0 .0 1) . 3组间肝 P45 0 3A酶 m RNA表达无明显差别 .结论　甘草酸对小鼠肝 P45 0 3A酶活性、蛋白含量及其 m RNA表达无明显影响 ;苯巴比妥钠是小鼠肝P45 0 3A酶诱导剂 ,其作用机制位于转录后水平 .AIM To study the effect of glycyrrhizin and phenobarbital pretreatment on the activity, content and mRNA expression of hepatic P450 3A enzyme in mice. Mice were given ig glycyrrhizin 50 mg·kg -1 ,phenobarbital 50 mg·kg -1 or equal amount of distilled water for 7 d, and the activity,protein content and mRNA expression of P450 3A enzyme in hepatic microsome were determined with MEGX test, ELISA and RT PCR method respectively. RESULTS The recovery of urinary MEGX and the content of P450 3A enzyme insignificantly differed between mice pretreated with glycyrrhizin and as normal control;but in mice administered ig phenobarbital the recovery of urinary MEGX increased by 23.4%( P <0.05). and the content of P450 3A enzyme increased by 43.4%( P <0.01).The mRNA of hepatic P450 3A enzyme showed high expression in the three group mice. CONCLUSION Glycyrrhizin pretreatment did not affect the activity. content and mRNA expression of hepatic P450 3A enzyme, but the administration of phenobarbital induced the enzyme post transcriptionally.

关 键 词：甘草酸 苯巴比妥钠 肝P4503A酶 

分 类 号：R975.5[医药卫生—药品] R966[医药卫生—药学]