冠心病猝死心肌中泛素活化酶UBE1、端粒酶的表达及意义  

Expression of UBE1,telomerase and its significance in sudden coronary death

作　　者：陈冰[1] 陈阳[2] 朱晓群[1] 陶香香[1] 何雷 

机构地区：[1]皖南医学院病理教研室,芜湖241001 [2]皖南医学院法医学系 [3]弋矶山医院病理科

出　　处：《山西医科大学学报》2013年第5期335-338,411,共5页Journal of Shanxi Medical University

基　　金：安徽高校省级自然科学研究基金资助项目(KJ2013B322);皖南医学院院中青年科研基金资助项目(WK201214)

摘　　要：目的探讨泛素活化酶UBE1、端粒酶在冠心病猝死心肌中的表达及意义。方法收集冠心病心源性猝死心肌28例、冠心病非心源性猝死心肌10例,10例正常心肌作为对照,应用免疫组化(EnVision二步法)技术及Image-Pro Plus 6.0图像分析软件检测各组UBE1、端粒酶表达的累积光密度(IOD)。结果 Image-Pro Plus 6.0图像分析软件显示UBE1在冠心病心源性猝死组、冠心病非心源性猝死组及正常对照组中累积光密度(IOD)的中位数(四分位数间距)依次为1182.77(952.82-1567.32)、892.69(603.38-1146.79)及833.52(752.74-873.54),端粒酶在以上三组中IOD的中位数(四分位数间距)依次为12 544.9(11 128.29-13 502.25)、6 368.07(3 648.35-12 485.16)及8 658.08(8 058.23-10 066.13)。UBE1、端粒酶在冠心病心源性猝死组中的累积光密度(IOD)显著高于冠心病非心源性猝死组及正常对照组,组间差异有统计学意义(P<0.05)。结论 UBE1和端粒酶在冠心病心源性猝死心肌中呈过表达,UBE1和端粒酶有望成为心肌早期损伤的标志物。Objective To investigate the expression and significance of ubiquitin activating enzyme（UBE1） and telomerase in the myocardium from sudden coronary death.Methods Myocardia from 28 cases of cardiac sudden coronary death,10 cases of non-cardiac sudden coronary death and 10 cases of normal controls were collected.Immunohistochemistry（EnVision two-step method） and ImagePro Plus 6.0 image analysis software were employed to detect the integrated optical density（IOD） of UBE1 and telomerase expression. Results The median（interquartile range） IOD of UBE1 was 1 182.77（952.82 - 1 567.32）,892.69（603.38 - 1 146.79） and 833.52（752.74 -873.54） in cardiac sudden coronary death group,non-cardiac sudden coronary death group and control group,respectively; the median（interquartile range） IOD of telomerase in the three groups was 12 544.9（11 128.29 - 13 502.25）,6 368.07 （3 648.35-12 485.16） and 8 658.08（8 058.23 -10 066.13）.respectively.IODs of UBE1 and telomerase in cardiac sudden coronary death group were significantly higher than those in non-cardiac sudden coronary death group and normal control group（P0.05）. Conclusion UBE1 and telomerase are overexpressed in the myocardia in sudden coronary death patients,and may be markers for diagnosing the early myocardial injury.

关 键 词：UBE1 端粒酶 免疫组织化学 猝死 

分 类 号：R541.4[医药卫生—心血管疾病]