TiO_2喷砂酸蚀处理对钛片表面人成骨细胞BMP-2表达水平的影响  

Effects of TiO_2 sandblasted and acid-etched titanium on expression of bone morphogenetic protein 2 in human osteoblasts

作　　者：陆斌[1] 李建武[1] 郭义[1] 杨艳[1] 

机构地区：[1]中国人民武装警察部队陕西省总队医院骨科,西安710054

出　　处：《山西医科大学学报》2013年第5期358-360,共3页Journal of Shanxi Medical University

基　　金：陕西省社会发展攻关计划资助项目(2012k16-09-01)

摘　　要：目的探讨钛片经过TiO_2喷砂酸蚀处理后对人成骨细胞系MG63细胞骨形态发生蛋白2(bone morphogenetic protein,BMP-2)表达水平的影响。方法将钛片分为3组进行处理:机械打磨组、喷砂组及喷砂酸蚀组,分别进行机械打磨、TiO_2喷砂和喷砂酸蚀处理。将人成骨细胞系MG63细胞接种于钛片表面,采用实时定量聚合酶链反应(real-time polymerase chain reaction,RT-PCR)、Western blot检测BMP-2 mRNA及蛋白表达水平。结果喷砂组及喷砂酸蚀组BMP-2 mRNA及蛋白水平增高,与机械打磨组相比差异有统计学意义(P<0.05),而喷砂组与喷砂酸蚀组之间差异无统计学意义(P>0.05)。结论使用经过TiO_2喷砂及喷砂酸蚀处理的钛片进行人成骨细胞培养可促进BMP-2表达。Objective To explore the effect of TiO_2 sandblasted and acid-etched titanium on the expression of bone morphogenetic protein 2（BMP-2） in human MG63 cells.Methods Titanium discs（15 mm diameter and 1 mm thickness） were divided into 3 groups; machine polished group,sandblasted group,sandblasted and acid-etched group.Titanium discs were treated with mechanical polishing, TiO_2 sandblasting,sandblasting and acid-etching in three groups,respectively.MG63 cells were cultured on the titanium.The mRNA and protein expression of BMP-2 in MG63 cells were analyzed by real-time polymerase chain reaction（RT-PCR） and Western blot. Results The mRNA and protein levels of BMP-2 were significantly higher in sandblasted group and sandblasted and acid-etched group than in machine polished group（P 0.05）.But the mRNA and protein levels of BMP-2 were not significantly different between sandblasted group and sandblasted and acid-etched group（P 0.05）.Conclusion After sandblasting and acid-etching,titanium could promote the expression of BMP-2 in human osteoblast.

关 键 词：钛片 喷砂酸蚀 成骨细胞 骨形态发生蛋白2 

分 类 号：R318.08[医药卫生—生物医学工程]