钉螺血淋巴细胞提取及其形态观察  

Extraction and Morphological Observation of Oncomelania hupensis Haemocytes

作　　者：许勇[1] 黄春兰[2] 周书林[2] 刘辉[3] 

机构地区：[1]皖南医学院医学一系 [2]皖南医学院医学寄生虫学教研室 [3]皖南医学院医学微生物学与免疫学教研室,芜湖241002

出　　处：《中国寄生虫学与寄生虫病杂志》2013年第3期211-213,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：安徽省自然科学基金(No.11040606M214);皖南医学院重点科研项目培育基金(No.WK2012Z06);皖南医学院大学生科研基金(No.WK2012S09)~~

摘　　要：利用软体组织破碎过滤法提取湖北钉螺(Oncomelania hupensis)血淋巴细胞,加入柠檬酸葡萄糖溶液作为抗凝剂,经吉氏染色和美兰染色后,用Motic B5计算机成像系统观察其形态。提取单只钉螺的血淋巴细胞,统计细胞数。结果共观察54个血淋巴细胞,可见3种形态:圆形有颗粒大细胞,直径(21.59～31.97)μm,细胞核呈卵圆形,有颗粒,核质比0.38±0.08,约占细胞总数35.95%(19/54);圆形无颗粒小细胞,直径(13.24～20.77)μm,细胞核呈卵圆形,无颗粒,核质比0.38±0.90,约占细胞总数51.63%(28/54);梭形细胞,大小(17.60～25.47)μm×(27.19～30.25)μm,细胞核呈长椭圆形,无颗粒,核质比0.44±0.20,约占细胞总数12.42%(7/54)。用过滤法提取单只钉螺血淋巴细胞均数约为19.46万个。该法是提取钉螺血淋巴细胞的一种有效方法。Haemocytes were collected from Oncomelania hupensis snails by using tissue disruption and filtration method, stained by Giemsa and methylene blue, respectively, and observed under microscope. Number of haemocytes from one snail was counted. Out of 54 haemocytes, 3 types of cells were found： big round cells with particles, small round cells with oval nuclei and spindle cells with oblong nuclei. The diameter of big round cells with particles and small round cells with oval nuclei was （21.59-31.97） and （13.24-20.77） μm, respectively. Spindle cells with oblong nuclei was about （17.60-25.47） μm×（27.19-30.25） μm. The nucleocytoplasmic ratio of the above three type cells was 0.38, 0.44 and 0.38, and occupied 35.95% （19/54）, 12.42% （28/54） and 51.63% （7/54）, respectively. About 194 600 haemocytes were filtered from one single snail. It means that this filtration method is an effective one to extract haemocytes from O. hupensis.

关 键 词：湖北钉螺 血淋巴细胞 过滤法 

分 类 号：R383.241[医药卫生—医学寄生虫学]