左旋精氨酸及硝基左旋精氨酸对内毒素休克大鼠转归的影响  

Effect of L-arginine and N-nitro-L-arginine on the outcome in endotoxic shock rat

作　　者：肖南[1] 刘韧[1] 田昆仑[1] 刘萍[1] 刁有芳[1] 陈惠孙[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所,重庆400042

出　　处：《创伤外科杂志》2000年第3期162-164,共3页Journal of Traumatic Surgery

摘　　要：目的 　 一氧化氮 (NitricOxide ,NO)在感染性休克中具有多种作用 ,但其对休克转归的影响目前仍不清楚。本研究用NO产生的前体物质L arg和其抑制剂NNLA从正反两个方面探讨其对内毒休克大鼠转归的影响。方法　实验大鼠分为生理盐水对照组 (NS)、内毒素 (10mg/kg ,ip)组 (ETX)、内毒素 +NNLA(4mg/kg ,ip)组、内毒素 +L arg(40mg/kg ,ip)组和内毒素 +NNLA +L arg组。上述处理后 ,测定大鼠不同时间的平均动脉压 (meanarterialpressure ,MAP)、血浆NO水平 (NO-2 /NO-3 )以及 2 4小时存活率。结果　内毒素休克大鼠 ,血浆NO-2 /NO-3 明显增加 ,同时MAP降低 ,NNLA虽可抑制NO产生 ,升高MAP ,但显著降低12小时和 2 4小时存活率 ;而L arg虽不能升高MAP ,但可使其维持稳定 ,且 2 4小时存活率明显提高。 结论　在本实验条件下 ,NO对内毒素休克可能有有利作用 ,抑制NO合成则对休克的转归不利。Objective Nitric oxide(NO)has several pathophysiological effects on septic shock,however,its effect on the outcome of septic shock is still contrasty.This study was to evaluate NO effect on the outcome in endotoxic shock rat by using L arg,the precursor of NO,and NNLA,the inhibitor of NO formation.Methods A total of 213 rats,weighing 180-230g,were subjected to this study. The animals were divided into normal saline control(NS),endotoxin(ETX,LPS 10mg/kg,ip),ETX+NNLA(4mg/kg,ip),ETX+L arg(40mg/kg,ip),and ETX+NNLA+L arg groups.MAP was monitored,24 hour survival rate and the level of plasma NO - 2/NO - 3,the stable metabolic end products of NO,were determined.Results Plasma NO - 2/NO - 3significantly increased and MAP gradually decreased after ETX administration.Although inhibition of NO production with NNLA could significantly increase MAP at early phase,it remarkably decrease 12 and 24 hour survival rate,Administration of L arg could,however,maintain MAP at late phase and significantly improve 24 survival rate.Conclusion NO may have beneficial effect on the outcome of endotoxic shock in the study and inhibition of NO production may be detrimental.

关 键 词：一氧化氮 内毒素休克 左旋精氨酸 硝基左旋精氨酸 

分 类 号：R631.405[医药卫生—外科学]