超声激励荧光微泡对兔乳腺癌转移淋巴结的释放作用  

Sonorelease of DiO-microspheres to the lymph nodes metastasis of rabbits VX2breast cancer

作　　者：乔璐[1] 朱梅[2] 高文宏[1] 张莉[1] 李露[1] 刘政[1] 

机构地区：[1]第三军医大学新桥医院超声科,重庆400037 [2]昆明医学院第一附属医院超声科,云南昆明650032

出　　处：《中国医学影像技术》2013年第6期871-874,共4页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金(30860269);云南省应用基础研究计划面上项目(C0620056Q)

摘　　要：目的观察超声激励荧光微泡空化对兔乳腺癌转移淋巴结的荧光释放作用。方法使用二甲基亚砜(DMSO)溶解绿色细胞膜荧光分子探针(DiO),抽取少量溶解液与脂质微泡混和,通过高速机械振荡制成荧光微泡。选取16只荷VX2乳腺癌的新西兰大白兔随机分成荧光微泡联合超声空化组和单纯荧光微泡组。荧光微泡联合超声空化组经瘤周皮下注射1ml荧光微泡并按摩,待瘤周引流淋巴结超声显影后,采用脉冲式治疗超声间歇辐照淋巴结5次,共30min;单纯荧光微泡组同样经瘤周皮下注射荧光微泡并按摩,但给予治疗超声假照。剖取淋巴结标本行冰冻组织切片,于激光共聚焦显微镜下观察淋巴结内的荧光分布情况,并定量分析荧光面积、累积光密度(IOD)及平均光密度(AOD)。结果荧光微泡联合超声空化组淋巴结的荧光面积、IOD和AOD均高于单纯荧光微泡组(P均<0.05)。结论荧光脂质微泡经瘤周注射不仅可进入兔乳腺癌模型的淋巴管使淋巴结显影,且可在治疗超声的激励作用下实现荧光物质在局部淋巴结高浓度释放。Objective To observe the sonorelease of fluresence microbubbles to the lymph nodes metastasis of rabbits VX2 breast cancer by uhrasound excitation. Methods DiO-labeled microbubbles were prepared with lipid microbubhles and mixed Dimethyl sulfoxide （DMSO） dissolved DiO. The fluorescence microbubbles were made by high speed mechanical agitation. Rabbits models of VX2 breast carcinoma were established by means of injection of tissue mass suspension and were divided into fluorescent mi crobubbles combined ultrasonic cavitation group and fluorescence of microbubbles group （each ,1= 8）. In fluorescent microbubbles combined ultrasonic cavitation group, the fluorescent microbubbles （total 1 ml） were injected subcutaneously around tumor and massaged to drainage of lymph nodes. The lymph nodes were exposured to pulse ultrasound intermittent treatment for 5 times, total 30 min. In fluorescence of microbubbles group, the same injection without ultrasound irradiation was performed. Laser confocal mi croscope was used to observe the deposition of green fluorescein in a small portion of frozen section lymph node tissues and to analyze the fluorescence of the area integrated optical density （IOD） and average optical density （AOD）. Results Compared with fluo- rescence of microbubbles group, the fluorescence area, IOD and AOD of lymph node were higher in fluorescent microbubbles combined ultrasonic cavitation group （all P〈0. 05）. Conclusion Flurescence microbubbles can not only enter lymph vessel to make lymph node development, but also deliver a high concentration of fluorescence microbubbles in the regional lymph node by low- pressure ultrasound in rabbit models.

关 键 词：微泡 空化 乳腺肿瘤 淋巴结 

分 类 号：R445.1[医药卫生—影像医学与核医学] R332[医药卫生—诊断学]