Che-1在创伤性脑损伤模型大鼠皮质中的表达及其与神经元的凋亡及轴突再生的关系  

Che-1 participates in the regulation of rat cortical neuronal apoptosis and axonal regeneration after traumatic brain injury via p53

作　　者：李爱红[1] 郭爱松[2] 陈鑫[1] 孙晓雷[3] 

机构地区：[1]南通大学附属医院神经内科,226001 [2]南通大学附属医院康复医学科,226001 [3]南通大学医学院

出　　处：《中华医学杂志》2013年第21期1664-1668,共5页National Medical Journal of China

摘　　要：目的探讨Che-1在创伤性脑损伤后大鼠皮质的表达变化及其与神经元凋亡和轴突再生的关系。方法64只雄性SD大鼠按随机数字表法分为正常组、假手术组和外伤组，其中外伤组又分为12h、1d、3d、5d、7d、14d6个时相组，每组8只。建立CCI颅脑损伤模型，使用Western印迹、免疫组化、免疫荧光、TUNEL标记等方法检测Che-1达变化，神经元凋亡，p53、Bax、active-caspase-3、GAP43的表达变化及与Che．1的定位关系。结果相比较于正常组和假手术组，外伤后Che-1表达量增加，其蛋白表达量于损伤后第3天达到峰值（0．817±0．022，P〈0．05），且与神经元凋亡和轴突再生相关。p53、Bax、active-caspase-3、GAP43在外伤后的表达变化与Che-1存在时间和定位相关性。结论创伤性脑损伤后Che-1表达增加，且通过p53参与外伤后神经元凋亡与轴突再生。Objective To explore the altered expressions of Che-1 in rat brain cortex and relative biological functions after traumatic brain injury. Methods According to a random number table, a total of 64 male Sprague-Dawley rats were divided into normal, sham and trauma group. Then the trauma group was further divided into 6 phase sub-groups （12 h, 1 d, 3 d, 5 d, 7 d, 14 d） （n =8 each）. The eranioeerebral injury （CCI） model was established to induce brain trauma at different timepoints. The examinations of Western blot and immunohistochemistry were performed to detect the expressions and diffusion changes of Che-1. Meanwhile the method of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling （TUNEL） was employed to examine neuronal apoptosis. Additionally, the association of Che-1 with p53, active-easpase-3 and GAP43 was tested with Western blot and immunofluoreseent staining. Results Compared with the normal and sham groups, the expression of Che-1 peaked at Day 3 post-injury （0.817 _＋ 0. 022, P 〈 0.05 ） and it was related with neuronal apoptosis. Moreover, the altered expressions of p53, GAP43 and active-caspase-3 were associated with the Level of Che-1. Conclusion The expression of Che-1 is elevated after brain trauma and may be involved in neuronal apoptosis and axonal regeneration through p53.

关 键 词：脑损伤 基因 p53 细胞凋亡 轴突 

分 类 号：R651[医药卫生—外科学] R-332[医药卫生—临床医学]