维生素D受体基因型多态性与绝经前后妇女的骨密度  

Relationship between the Polymorphism of Vitamin D Receptor Gene and Bone Mineral Density in Pre- and Postmenopausal women

作　　者：张清学[1] 王文军[1] 邝健全[1] 申欢莲[2] 黄宏兴[2] 蒋宁一[3] 

机构地区：[1]中山医科大学孙逸仙纪念医院妇产科 [2]广州中医药大学附属骨伤科医院 [3]中山医科大学孙逸仙纪念医院核医学科,广东广州510120

出　　处：《中山医科大学学报》2000年第5期376-379,共4页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：广东省卫生厅"五个一科教兴医工程"重点项目!(编号E0 0 0 0 96 0 92 );中山医科大学科研基金!资助 (B0 0 0 0 9712 4)

摘　　要：【目的】观察维生素D受体 (VDR)基因型与女性峰值骨密度以及绝经后骨质疏松的关系。【方法】收集 184例妇女 ,其中绝经后无骨折妇女 78例、绝经后有脆性骨折的病人 34例、围绝经期妇女 2 0例和年轻健康妇女 5 2例 ,用双能X线吸收骨密度仪 (DEXA)测量其骨密度 ,聚合酶链反应 限制性片段长度多态 (PCR RFLP)的方法来分析维生素D受体基因型。【结果】 184例妇女中 ,基因型BB、Bb和bb所占比例分别为 7 6 % (14/184)、45 1% (83/184)和 47 2 % (87/184) ;年轻健康妇女各基因型的峰值骨密度无明显差别 ;绝经后妇女各基因型的骨密度和骨质疏松的百分比也无明显差异。【结论】维生素D受体基因型与女性峰值骨密度和绝经后骨质疏松无明显联系。Objective To observe the relationship between Vitamin D receptor (VDR) genotype and bone mineral density in pre- and postmenopausal women. Methods Seventy-eight postmenopausal women without fragile fractures, 34 postmenopausal women with fragile fractures, 20 peri-menopausal women with climacteric symptoms, and 52 healthy young women (aged 25～35 years) were enrolled the study. Bone mineral density was measured by dual energy X-ray absorptiometry (DEXA) at lumbar spine, proximal femur, forearm and total body. We also examined the restriction fragment length polymorphism of the polymerase chain reaction product (PCR-RFLPs) of the VDR gene with BsmI enzyme (B, absence, b, presence of cut site) in these women. Result In 184 women, the distribution of BsmI RFLPs was as follow: BB, 14(7 6%); Bb, 83(45 1%); bb, 96(47 2%); respectively. The bone mineral density in healthy young women was not significantly different among the three genotypes groups. The bone mineral density in postmenopausal women and the ratio of postmenopausal osteoporosis were also not significantly different among the three genotypes groups. Conclusion These data indicated no significant effects of the VDR genotypes defined by BsmI on female peak bone mineral density and postmenopausal osteoporosis.

关 键 词：维生素D受体基因型 骨密度 绝经后 骨质疏松 

分 类 号：R681.1[医药卫生—骨科学]