巴泰病毒云南株生物学性状研究  

Biological characters of Batai virus isolated from Yunnan Province

作　　者：章域震[1] 张海林[1] 杨卫红[1] 冯云[1] 

机构地区：[1]云南省地方病防治所/云南省病毒立克次体研究中心,大理671000

出　　处：《中国人兽共患病学报》2013年第6期527-532,共6页Chinese Journal of Zoonoses

基　　金：国家自然科学基金项目(No.30560142)~~

摘　　要：目的掌握巴泰(Batai)病毒生物学特性,为巴泰病毒的监测和调查研究提供科学依据。方法用分离自云南省菲律宾按蚊(Anopheles philippinensis)的巴泰病毒LC92-4株,采用细胞培养、小白鼠感染、血凝抑制、间接免疫荧光、空斑试验以及病理检测和电镜观察等方法进行生物学特性试验。结果巴泰病毒可引起<4周龄小白鼠发病死亡。感染鼠脑病毒颅内、皮下和腹腔接种2周龄小白鼠的LD50分别为5.67logLD50/0.025mL、5.3logLD50/0.03mL和4.5logLD50/0.03mL。感染病毒鼠的心、肝、脾、肺、肾病毒悬液颅内接种2周龄小白鼠,LD50分别为3.75、4.25、2.25、3.0、2.25logLD50/0.025mL。感染发病小白鼠脑、心、肝、脾、肺、肾均发现病理改变。感染鼠的脑组织经电镜检测发现圆形有包膜病毒颗粒,大小约82nm。巴泰病毒能在BHK21和Vero细胞产生明显病变,并可产生空斑;在BHK21细胞上,空斑滴度为6.04logPFU/mL;在C6/36细胞上不产生病变。感染病毒鼠脑接种BHK21和Vero细胞,TCID50分别为5.50logTCID50/0.025mL和5.00logTCID50/0.025mL。在pH5.75～7.0条件下,巴泰病毒不具有与鸽、鹅、鸡、鸭、人O型和绵羊红血球发生凝集的特性;空斑减少中和试验检测云南省357份人血清,阳性2份,中和抗体滴度均为1∶10。结论乳小白鼠和幼年小白鼠对巴泰病毒高度易感。BHK21和Vero细胞对巴泰病毒敏感,可用于病毒分离培养和空斑测定。建立的免疫荧光和空斑减少中和试验具有特异性,可用于巴泰病毒抗体检测。应进一步开展该病毒的分布及其与疾病关系的研究。The purpose of this study is to understand the biological characteristics of Batai virus isolated from Anopheles philippinensis in Lancang County, Yunnan Province. The mice younger than 4 weeks were susceptible to Batai virus. The titers of LDs0 in 2-weeks old mice inoculated via subcutaneous, intraperitoneal and intracerebral routes were 4. 5 logLDs0/ 0.03 mL, 5.3 logLDs0 /0. 03 mL and 5. 67 IogLDs0/0. 025 mL, respectively. The tissue suspensions of heart, liver, spleen, lung and kidney of morbid mice were inoculated with 2-weeks old mice and the titers of LDs0 were 3.75, 4.25, 2.25, 3.0, and 2.25 logLDs0/0. 025 mL. Pathological changes were found in the organs. Virus particles were found in the infected mice brain via electron microscope detection and their size were about 82 nm. CPE was observed in BHK21 and Vero cells, but not in C6/ 36 cell. The titers of TCIDs0 in BHK and Vero were 5.50 logTCIDs0/0. 025 mL and 5.00 logTCIDs0/0. 025 mL respectively. PFU in BHK21 was 6.04 logPFU/mL. As buffer solutions were chosen ranging from pH5.75-pH7.0, no hemagglutinative character was observed with the six kinds of red blood cells. With plaque reduction neutralization test, 2 positive sera among 357 human sera from Yunnan Province were detected with the same titer of 1 ： 10. Our results show that the suckling and in rant mice are highly susceptible to BATV. BHK21 and Vero ceils, both of which are susceptible to BATV, can be employed for isolating this virus and plaque assay. Both of IFA and PRNT are applicable for detecting the antibody of this virus.

关 键 词：巴泰病毒 动物易感性 细胞敏感性 间接免疫荧光试验 空斑试验 空斑减少中和试验 

分 类 号：R373.3[医药卫生—病原生物学]