浙江省甲型H1N1流感病毒血凝素基因序列分析  

Sequence analysis of hemagglutinin gene of pandemic influenza A (H1N1) viruses in Zhejiang Province

作　　者：李敏红[1] 周敏[1] 茅海燕[1] 张严峻[1] 卢亦愚[1] 陈寅[1] 

机构地区：[1]浙江省疾病预防控制中心,杭州310051

出　　处：《中国人兽共患病学报》2013年第6期566-569,共4页Chinese Journal of Zoonoses

基　　金：浙江省自然科学基金(No.Y207819);浙江省医药卫生科学研究基金(No.2010KYB031)资助~~

摘　　要：目的分析浙江省2009-2011年甲型H1N1流感病毒血凝素基因(HA)序列进化特征。方法提取浙江省2009-2011年8株甲型H1N1流感病毒基因组RNA,RT-PCR扩增血凝素基因,测序并拼接出ORF;从GenBank数据库下载2009-2012年国内外甲型H1N1流感病毒HA基因序列,选择20株作为代表进行分析。采用MEGA软件对其进行种系发生树的构建。结果扩增并测序获得8株甲型H1N1流感病毒1 701bp的HA基因ORF,与国内外甲型H1N1流感病毒HA序列比对后发现所有序列的同源性均较高,2009年和2010年浙江省3株病毒HA与北美早期毒株A/California/04/2009(H1N1)同源性达到99.4%～99.5%,2011年浙江省5株病毒HA与A/California/04/2009(H1N1)同源性为98.5%～99.1%。在1株来自重症患者的毒株中发现了D222G突变。结论与早期毒株相比,虽然浙江省2011年的甲型H1N1流感病毒血凝素基因累积了更多的变异,但所有毒株的同源性达到98.5%以上。血凝素中D222G突变可能与重症甲型H1N1流感病毒感染相关。The aim was to analyze the evolutionary characteristics of hemagglutinin gene of pandemic influenza A （H1N1） virus isolated from Zhejiang Province during 2009 and 2011. Viral RNA was extracted from 8 influenza A （HIN1） vi rus strains. Hemagglutinin gene was ampl.ified by RT-PCR and the ORF sequence was joined together using sequencing data. Hemagglutinin genes from 20 representative domestic and overseas strains during 2009 to 2012 were downloaded from GenBank database for analysis. All the sequences were used for construction of phylogenetic tree by MEGA software. The 1 701 bp ORF sequences of hemagglutinin gene were obtained by analyzing sequencing results of the 8 strains. After alignment, high homolo gy were observed among all the HA genes originated all over the world. The homology between 3 strains from Zhejiang Prov ince during 2009 and 2010 and the representative strain of A/California/04/2009 （H1N1） from North America was as high as 99.4%--99. 5%. The homology between 5 strains from Zhejiang Province isolated in 2011 and the A/California/04/2009 （H1N1） was 98.5%--99. 1%. A D222G mutation in hemagglutinin protein was observed in the A/Zhejiang-Yiwu/ll/2009 （H1N1） strain isolated from a patient with severe influenza A （HIN1） infection. Although more mutations were accumulated in HA genes in the strains from Zhejiang Province in 2011 comparing with strains from early stage of the pandemic, high ho mology of more than 98.5 % was observed in all the HA genes. A D222G mutation in HA might be related to the severe ill ness.

关 键 词：甲型H1Nl流感病毒 血凝素 序列分析 种系发生树 

分 类 号：R373.1[医药卫生—病原生物学]