巨噬细胞吞噬黑素化与非黑素化马尔尼菲青霉的电镜观察  

TEM observations of macrophages phagocytizing melanized and non-melanized Penicillium marneffei yeast cells

作　　者：谭伟芬[1] 梁伶[1] 刘栋华[1] 韦立莉[1] 

机构地区：[1]广西医科大学第一附属医院皮肤性病科,南宁530021

出　　处：《中国人兽共患病学报》2013年第6期575-578,586,共5页Chinese Journal of Zoonoses

基　　金：国家自然科学基金项目(No.81160191);广西自然科学基金项目(No.2011GXNsFA018255)联合资助~~

摘　　要：目的通过透射电镜(TEM)比较巨噬细胞吞噬黑素化与非黑素化马尔尼菲青霉酵母相孢子的区别,初步探讨黑素在马尔尼菲青霉致病机制中所发挥的作用。方法将马尔尼菲青霉酵母相孢子与Ana-1小鼠巨噬细胞系共同培养,分为处理组A、B、C和D组:A组加入干扰素-γ及L-DOPA,B组仅加入干扰素-γ,C组仅加入L-DOPA,D组不加干扰素-γ及L-DOPA,设置基础对照组E为加入L-DOPA培养的巨噬细胞。7d后离心收集各组巨噬细胞和孢子,透射电镜观察并比较其超微形态变化。结果无论是否加入干扰素-γ均可观察到巨噬细胞吞噬马尔尼菲青霉酵母相孢子现象。加入L-DOPA培养的A、C组巨噬细胞内外的酵母细胞壁内侧面均产生了颗粒状电子致密物,一个巨噬细胞吞噬孢子数目为2～6个。未加入L-DOPA培养的B、D组巨噬细胞内外的酵母细胞壁内侧面无颗粒状电子致密物,一个巨噬细胞吞噬孢子数目为10～25个。结论 L-DOPA可以促进马尔尼菲青霉酵母相孢子黑素颗粒的合成,黑素化培养的马尔尼菲青霉酵母相孢子逃避巨噬细胞吞噬的能力增强。To observe the interactions of melanized and non-melanized P. marneffei with macrophages by TEM and to investigate the role of melanin in the pathogenicity of P. marneffei, yeast cells of P. marneffei were cultured with the Ana-1 mouse macrophage cell line for 7 days, which were divided into A, B, C, and D groups. Group A was cultured with IFNy and L-DOPA, group B was cultured with IFN-7, group C was cultured with L-DOPA, and group D was cultured with no IFN nor L-DOPA. The Ana-1 mouse macrophage cells were cultured alone without IFN 7 or yeast cells as blank control group E. The macrophage cells and the yeast cells were collected for examination by transmission electron microscopy 7 days after cul ture. The phagocytosis of yeast cells by the macrophages was observed regardless of the macrophages activated with IFN 7 or not. It was found that the yeast cells in group A and C cultured with L-DOPA produced granular electron-dense material at the inside cell wall, and phagocytosis of 2-6 yeast cells by every macrophage was observed. The yeast cells in group B and D cul- tured without L-DOPA did not produce granular electron-dense material at the inside cell wall, and phagocytosis of 10 25 yeast cells by every macrophage was observed. Results suggest that L-DOPA may help the yeast cells of P. marneffei producing melanin granules, and the melanized yeast cells increase the abilities of evading from macrophages phagocytosis.

关 键 词：马尔尼菲青霉 巨噬细胞 黑素 

分 类 号：R379[医药卫生—病原生物学]